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I used ab53132 and have not been able to detect the target protein via IHC-P. I tried 1 hr incubation of primary and 10' heat retrieval at pH6 and got no staining; tried overnight incubation with primary at 4 deg C and 20' retrieval (boiling water bath) and got really high background.
Asked on Feb 13 2012
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab111609.
As we discussed, this antibody is backordered 2-3 weeks. To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Feb 13 2012