Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT2 (phospho Y690) antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citric acid (pH 6.0) for 10 min at ~100¯C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.02% triton
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.01% triton and 2% serum
Dilution
1/50

Secondary antibody

Concentration
5 µg/ml
Name
Non-Abcam antibody was used: biotinilated anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
streptavidin alexa 594 was added as last step of detection in concentration of 1:100.

Mr. Shachar Dagan

Verified customer

Submitted Mar 10 2014

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