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Signal Transduction Signaling Pathway Nuclear Signaling STATs
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Anti-STAT3 antibody [9D8] (ab119352)

  • Datasheet
Reviews (11)Q&A (1)References (107)

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Western blot - Anti-STAT3 antibody [9D8] (ab119352)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [9D8] (ab119352)
  • Flow Cytometry - Anti-STAT3 antibody [9D8] (ab119352)
  • Western blot - Anti-STAT3 antibody [9D8] (ab119352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
  • Western blot - Anti-STAT3 antibody [9D8] (ab119352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
  • Immunoprecipitation - Anti-STAT3 antibody [9D8] (ab119352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

Key features and details

  • Mouse monoclonal [9D8] to STAT3
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Non human primates, African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-STAT3 antibody [9D8]
    See all STAT3 primary antibodies
  • Description

    Mouse monoclonal [9D8] to STAT3
  • Host species

    Mouse
  • Specificity

    Detects STAT3 from Human, murine, monkey and rat samples.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to amino acids 665-770 of Human STAT3.

  • Positive control

    • HepG2, 293T, Jurkat, A431, U2OS, MCF7, A549, K562, NIH3T3, C2C12, and NRK whole cell lysates; U2OS lysate from STAT3 SMART pool siRNA transfected; HeLa cells; Human pancreas, brain tumour, colon cancer and stomach cancer tissues Flow Cyt: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    9D8
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Assay kits

    • STAT3 Transcription Factor Assay Kit (Colorimetric) (ab207229)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
    • C2C12 whole cell lysate (ab7182)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
    • A549 whole cell lysate (ab7910)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human STAT3 protein (ab43618)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
Flow Cyt
Use 0.1µg for 106 cells.
WB (2)
1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP
Use at 2 µg/mg of lysate.
IHC-P (5)
1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.
Notes
Flow Cyt
Use 0.1µg for 106 cells.
WB
1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP
Use at 2 µg/mg of lysate.
IHC-P
1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.

Target

  • Function

    Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • Tissue specificity

    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in disease

    Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Target information above from: UniProt accession P40763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6774 Human
    • Entrez Gene: 20848 Mouse
    • Entrez Gene: 25125 Rat
    • Omim: 102582 Human
    • SwissProt: P40763 Human
    • SwissProt: P42227 Mouse
    • SwissProt: P52631 Rat
    • Unigene: 463059 Human
    • Unigene: 249934 Mouse
    • Unigene: 473190 Mouse
    • Unigene: 10247 Rat
    see all
  • Alternative names

    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • ADMIO antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Images

  • Western blot - Anti-STAT3 antibody [9D8] (ab119352)
    Western blot - Anti-STAT3 antibody [9D8] (ab119352)
    All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution

    Lane 1 : U2OS lysate from non-targeting control
    Lane 2 : U2OS lysate from STAT3 SMART pool siRNA transfected

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa



    4-20% Tris-HCl polyacrylamide gel
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [9D8] (ab119352)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT3 antibody [9D8] (ab119352)

    Immunofluorescent analysis of STAT3 using ab119352 at 1/100 dilution (shown in green) in HeLa cells (formalin fixed cells permeabilized with 0.1% Triton X-100) using a DyLight 488 goat-anti-mouse secondary antibody (ab96879) at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.

  • Flow Cytometry - Anti-STAT3 antibody [9D8] (ab119352)
    Flow Cytometry - Anti-STAT3 antibody [9D8] (ab119352)

    Overlay histogram showing HeLa cells stained with ab119352 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119352, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (ab37355, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-STAT3 antibody [9D8] (ab119352)
    Western blot - Anti-STAT3 antibody [9D8] (ab119352)
    All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : U2OS cell lysate
    Lane 6 : MCF7 cell lysate
    Lane 7 : A549 cell lysate
    Lane 8 : K562 cell lysate
    Lane 9 : COS7 cell lysate
    Lane 10 : NIH3T3 cell lysate
    Lane 11 : C2C12 cell lysate
    Lane 12 : NRK cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa



    4-20% Tris-HCl polyacrylamide gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

    ab119352, at 1/1600 dilution, staining STAT3 in Human pancreas tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

  • Western blot - Anti-STAT3 antibody [9D8] (ab119352)
    Western blot - Anti-STAT3 antibody [9D8] (ab119352)
    Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution + HepG2 total lysate at 25 µg

    Secondary
    goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa



    4-20% Tris-HCl polyacrylamide gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

    ab119352, at 1/1600 dilution, staining STAT3 in Human brain tumor tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

  • Immunoprecipitation - Anti-STAT3 antibody [9D8] (ab119352)
    Immunoprecipitation - Anti-STAT3 antibody [9D8] (ab119352)
    Immunoprecipitation of STAT3 was performed on HepG2 cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of ab119352 overnight at 4°C. The immune-complex was captured, washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1% Tween for at least 1 hour. Membranes were then probed with ab119352 at a dilution of 1/5000 overnight at 4°C. Membranes were washed in TBST and probed with IP detection reagent at a dilution of 1/2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

    ab119352, at 1/1600 dilution, staining STAT3 in Human colon cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 antibody [9D8] (ab119352)

    ab119352, at 1/1600 dilution, staining STAT3 in Human stomach cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (107)

    Publishing research using ab119352? Please let us know so that we can cite the reference in this datasheet.

    ab119352 has been referenced in 107 publications.

    • Chen S  et al. N-myc Downstream-Regulated Gene 2 (NDRG2) Promotes Bone Morphogenetic Protein 2 (BMP2)-Induced Osteoblastic Differentiation and Calcification by Janus Kinase 3 (JAK3)/Signal Transducer and Activator of Transcription 3 (STAT3) Signaling Pathway. Med Sci Monit 26:e918541 (2020). PubMed: 31911574
    • Song X  et al. EGFR Promotes the Development of Triple Negative Breast Cancer Through JAK/STAT3 Signaling. Cancer Manag Res 12:703-717 (2020). PubMed: 32099467
    • Deng QF  et al. Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis. J Cancer 11:3667-3674 (2020). PubMed: 32284763
    • Tang H  et al. Delayed recanalization after MCAO ameliorates ischemic stroke by inhibiting apoptosis via HGF/c-Met/STAT3/Bcl-2 pathway in rats. Exp Neurol 330:113359 (2020). PubMed: 32428505
    • Liu G  et al. Circ_0038467 regulates lipopolysaccharide-induced inflammatory injury in human bronchial epithelial cells through sponging miR-338-3p. Thorac Cancer 11:1297-1308 (2020). PubMed: 32181994
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 12 Abreviews or Q&A

    ChIP abreview for Anti-STAT3 antibody [9D8]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Glioma cell lines U87MG and U251MG)
    Negative control
    non specific IP by IgG
    Specification
    Glioma cell lines U87MG and U251MG
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde in PBS at room
    Positive control
    Discontinued STAT3 antibody sc482X OSMR promoter, a gene previously reported to be regulated by STAT3 (eg/ PMID: 29142070)
    Read More

    Dr. Julie Thoms

    Verified customer

    Submitted Dec 24 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate ph 6
    Permeabilization
    No
    Specification
    brain tissue
    Blocking step
    Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Herr Dr. Markus Kipp

    Verified customer

    Submitted Aug 21 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [9D8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (tumor)
    Permeabilization
    No
    Specification
    tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [9D8]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (artery)
    Permeabilization
    No
    Specification
    artery
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    tumor xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 antibody [9D8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    stomach
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2018

    Western blot abreview for Anti-STAT3 antibody [9D8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    22 µg
    Specification
    Lung
    Blocking step
    Odyssey TBS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
    Read More

    Pete Serbedzija

    Verified customer

    Submitted Jul 28 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-STAT3 antibody [9D8]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Sample
    Mouse Tissue sections (mouse embryonic spinal cord E11)
    Specification
    mouse embryonic spinal cord E11
    Permeabilization
    Yes - 0.25%TritonX100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 28 2014

    1-10 of 12 Abreviews or Q&A

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