Key features and details
- Rabbit polyclonal to STAT3 (phospho S727)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-STAT3 (phospho S727) antibody
See all STAT3 primary antibodies
DescriptionRabbit polyclonal to STAT3 (phospho S727)
Specificityab131103 detects endogenous levels of STAT3 only when phosphorylated at serine 727.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- HeLa and HepG2 cell extract treated with PMA; HeLa cells; Human breast carcinoma tissue
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131103 was purified by affinity-chromatography using epitope-specific phosphopetide. Non-phospho specific antibodies were removed by chromatography using non-phosphopetide.
Our Abpromise guarantee covers the use of ab131103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 88 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionSignal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in diseaseHyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
Autoimmune disease, multisystem, infantile-onset
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
- Information by UniProt
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
All lanes : Anti-STAT3 (phospho S727) antibody (ab131103) at 1/500 dilution
Lane 1 : HeLa cell extract untreated
Lane 2 : HeLa cell extract treated with PMA
Lane 3 : HepG2 cell extract untreated
Lane 4 : HepG2 cell extract treated with PMA
Predicted band size: 88 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling STAT3 (phospho S727) with ab131103 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Immunofluorescence analysis of methanol-fixed HeLa cells labelling STAT3 (phospho S727) with ab131103 at 1/100 dilution.
ab131103 has been referenced in 2 publications.
- Feng J et al. Blocking STAT3 by pyrvinium pamoate causes metabolic lethality in KRAS-mutant lung cancer. Biochem Pharmacol 177:113960 (2020). PubMed: 32298693
- Gu J et al. Human CD39hi regulatory T cells present stronger stability and function under inflammatory conditions. Cell Mol Immunol 14:521-528 (2017). PubMed: 27374793