Overview

  • Product name

    Anti-STAT3 (phospho S727) antibody
    See all STAT3 primary antibodies
  • Description

    Rabbit polyclonal to STAT3 (phospho S727)
  • Host species

    Rabbit
  • Specificity

    ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, WB, ELISA, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
    Database link: P40763
    (Peptide available as ab43618, ab82053)

  • Positive control

    • IHC: Human breast carcinoma tissue. WB: HeLa and COS7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20950279
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Recombinant Human STAT3 protein (ab43618).
ELISA 1/10000.
IP Use at an assay dependent concentration.

Target

  • Function

    Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • Tissue specificity

    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in disease

    Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • ADMIO antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Images

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)

    Lane 1 : HeLa cells extract
    Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
    Lane 3 : HeLa cells extract with Synthesized phosphopeptide

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

  • ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab30647) at 1/500 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : COS7 cell lysate with phospho peptide

    Predicted band size: 88 kDa

  • PFA-fixed frozen rat liver tissue stained for STAT3 (phospho S727) using ab30647 at 1/1000 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 donkey anti-rabbit.

    See Abreview

References

This product has been referenced in:

  • Dong Z & Wang Y LncRNA BLACAT1 accelerates the proliferation and migration of osteosarcoma cells through regulating STAT3. Pathol Res Pract 215:571-579 (2019). Read more (PubMed: 30665678) »
  • Chen PJ  et al. microRNA-874 inhibition targeting STAT3 protects the heart from ischemia-reperfusion injury by attenuating cardiomyocyte apoptosis in a mouse model. J Cell Physiol 234:6182-6193 (2019). Read more (PubMed: 30370578) »
See all 27 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
45 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Lung)
Specification
Lung
Treatment
Basel conditions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Abcam user community

Verified customer

Submitted Oct 22 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 11 2012

Answer

Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum, I recommend to choose a color in the yellow spectrum to go with it.

These two secondaries will fit this description:

ab96920 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=96920).

ab98499 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98499).

Please make sure that the instrument you are planning to use has the right filter sets and lasers to be able to measure all three colors together.

I wish you good luck with your experiments.

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