Key features and details
- Rabbit polyclonal to STAT3 (phospho S727)
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-STAT3 (phospho S727) antibody
See all STAT3 primary antibodies
DescriptionRabbit polyclonal to STAT3 (phospho S727)
Specificityab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityProtein A purified
Purification notesab30647 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab30647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Recombinant Human STAT3 protein (ab43618).|
FunctionSignal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in diseaseHyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
Autoimmune disease, multisystem, infantile-onset
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
- Information by UniProt
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All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)
Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with Synthesized phosphopeptide
Predicted band size: 88 kDa
Observed band size: 88 kDa
Immunohistochemical analysis of human uterus tissue stained with ab30647 at 1/200 dilution at 4oC overnight. The negative control is stained with secondary antibody only.
Immunohistochemical analysis of human lung tissue stained with ab30647 at 1/200 dilution at 4oC overnight. The negative control is stained with secondary antibody only.
All lanes : Anti-STAT3 (phospho S727) antibody (ab30647) at 1/500 dilution
Lane 1 : COS7 cell lysate
Lane 2 : COS7 cell lysate with phospho peptide
Predicted band size: 88 kDa
ab30647 has been referenced in 42 publications.
- Su X et al. miR-613 suppresses migration and invasion in esophageal squamous cell carcinoma via the targeting of G6PD. Exp Ther Med 19:3081-3089 (2020). PubMed: 32256796
- Wang N et al. Yeast ß-D-glucan exerts antitumour activity in liver cancer through impairing autophagy and lysosomal function, promoting reactive oxygen species production and apoptosis. Redox Biol 32:101495 (2020). PubMed: 32171725
- Gao F et al. MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL. Braz J Med Biol Res 53:e9292 (2020). PubMed: 32321151
- Ma F et al. Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway. Theranostics 10:7730-7746 (2020). PubMed: 32685016
- Shen L et al. CircRNA-0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR-107. Mol Med Rep 19:3923-3932 (2019). PubMed: 30864721