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Thank you for your reply.
I’m going to use a coctail which contains the following antibodies: AB30647, AB25533 and AB25024. So I’m looking for secondary antibody to AB30647, which will be in one tube with AB25024 and AB25533. Do you have such a secondary antibody? Which secondary antibody do you recommend?
I would appreciate a reply at your earliest convenience.
Asked on Feb 02 2012
Thank you for your inquiry.
I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.
Since you are already using fluorescence in the green and red spectrum, I recommend to choose a color in the yellow spectrum to go with it.
These two secondaries will fit this description:
ab96920 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)
Click here (or use the following: https://www.abcam.com/index.html?datasheet=96920).
ab98499 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)
Click here (or use the following: https://www.abcam.com/index.html?datasheet=98499).
Please make sure that the instrument you are planning to use has the right filter sets and lasers to be able to measure all three colors together.
I wish you good luck with your experiments.
Answered on Feb 02 2012