Application
Western blot
Loading amount
45 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Lung)
Specification
Lung
Treatment
Basel conditions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBS-T
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: ~80 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
protease inhibitor cocktail tablet used in the cell lysis buffer (cell signaling)
Abcam user community
Verified customer
Submitted Oct 22 2013