Name: Anti-STAT3 (phospho Y705) antibody [EP2147Y]
Brand: RabMAb
SKU: ab76315
Rating: 4 (14 reviews)

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Primary

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This product Rabbit Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)
WB, IP, IHC-P, Dot blot, ICC/IF

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-STAT3 (phospho Y705) antibody [EP2147Y]
See all STAT3 primary antibodies
Description

Rabbit monoclonal [EP2147Y] to STAT3 (phospho Y705)
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-P, Dot blot, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human STAT3 aa 650-750 (phospho Y705). The exact sequence is proprietary.
Database link: P40763
(Peptide available as ab179551)
Positive control
- WB: HeLa cell lysate treated with alpha-interferon. IHC-P: Human colon carcinoma, kidney and thyroid carcinoma tissues and mouse spleen tissue ICC/IF: HeLa cells treated with alpha-interferon. IP: A431 cells treated with EGF.
General notes

A trial size is available to purchase for this antibody.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2147Y
Isotype

IgG
Research areas

Alternative Versions
Assay kits
- STAT3 Transcription Factor Assay Kit (Colorimetric) (ab207229)
Compatible Secondaries
Immunizing Peptide (Blocking)
- STAT3 (phospho Y705) peptide (ab179551)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- A431 nuclear extract lysate (EGF treated) (ab14635)
Recombinant Protein
- Recombinant Human STAT3 protein (ab43618)
Related Products

Our Abpromise guarantee covers the use of ab76315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2000 - 1/20000. Predicted molecular weight: 88 kDa.Can be blocked with STAT3 (phospho Y705) peptide (ab179551).
IP		1/20.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
Dot blot		1/1000.
ICC/IF		1/500.

Function

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
Tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease

Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
Autoimmune disease, multisystem, infantile-onset
Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications

Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Cellular localization

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
Target information above from: UniProt accession P40763 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P40763 Human
- SwissProt: P42227 Mouse
- SwissProt: P52631 Rat
- Unigene: 463059 Human
- Unigene: 249934 Mouse
- Unigene: 473190 Mouse
- Unigene: 10247 Rat
see all
Alternative names
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
- ADMIO antibody
- APRF antibody
- AW109958 antibody
- DNA binding protein APRF antibody
- FLJ20882 antibody
- HIES antibody
- MGC16063 antibody
- Signal transducer and activator of transcription 3 (acute phase response factor) antibody
- Signal transducer and activator of transcription 3 antibody
- STAT 3 antibody
- Stat3 antibody
- STAT3_HUMAN antibody
see all

Western blot - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

All lanes : Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315) at 1/20000 dilution (purified)

Lane 1 : Un-treated HeLa (Human epithelial cell line from cervix adenocarcinoma )cell lysate
Lane 2 : HeLa cell lysate - treated with IFN-a

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling STAT3 (phospho Y705) with purified ab76315 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) +/- IFN-α (50 ng/mL, 5 minutes) cells labelling STAT3 (phospho Y705) with ab76315 at 1/500 (4.3 μg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labeling STAT3 (phospho Y705) with ab76315 at 1/100 (nuclear staining). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labeling STAT3 (phospho Y705) with unpurified ab76315 at 1/100.
Dot Blot - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Dot blot analysis of STAT3 single phospho peptide pY705 (lane 1) and STAT3 non-phospho peptide (lane 2) with ab76315 at 1/1000. Blocking and dilution buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue using untreated (left) or alkaline phosphatase-treated (right) labeling STAT3 (phospho Y705) with ab76315 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H& L (HRP) (ab97051) at 1/500 dilution.

Counter stained with Hematoxylin.
Immunoprecipitation - Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

ab76315 (purified) at 1/30 immunoprecipitating STAT3 (phospho Y705) in A431 (Human epidermoid carcinoma cell line) cell lysate treated with EGF. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Li YM et al. Alterations of the Immunologic Co-Stimulator B7 and TNFR Families Correlate with Hepatocellular Carcinoma Prognosis and Metastasis by Inactivating STAT3. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30609841) »
Dolatabadi S et al. JAK-STAT signalling controls cancer stem cell properties including chemotherapy resistance in myxoid liposarcoma. Int J Cancer N/A:N/A (2019). Read more (PubMed: 30650179) »

See all 180 Publications for this product

Publishing research using ab76315? Please let us know so that we can cite the reference in this datasheet.

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1-10 of 16 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (peripheral blood CD19+ B cells)

Loading amount

200 µg

Specification

peripheral blood CD19+ B cells

Treatment

(1) Untreated (2) 100 ng recombinant hIL-10 for 24hours

Gel Running Conditions

Reduced Denaturing (10 % SDS-PAGE)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 07 2010

Application

Western blot

Sample

Human Cell lysate - whole cell (Hela)

Gel Running Conditions

Reduced Denaturing (4-12% Bis Tris)

Loading amount

10 µg

Treatment

IFN alpha

Specification

Hela

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 04 2019

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (brain)

Antigen retrieval step

None

Specification

brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

Fixative

Paraformaldehyde

Dr. Yona Goldshmit

Verified customer

Submitted Nov 29 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (lung)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TE pH9

Permeabilization

Specification

lung

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

10% normal buffered formalin

Abcam user community

Verified customer

Submitted Feb 05 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (stomach tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TE pH9

Permeabilization

Specification

stomach tumor

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

10% normal buffered formalin

Abcam user community

Verified customer

Submitted Feb 05 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6

Permeabilization

Specification

liver

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

10% normal buffered formalin

Abcam user community

Verified customer

Submitted Feb 05 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA 70C 2hr

Permeabilization

Specification

Colon

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jan 23 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Colon

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Dec 28 2017

Application

ELISA

Sample

Human Cell (HEK293 cells stably overexpressing the human Lepti)

Specification

HEK293 cells stably overexpressing the human Lepti

Blocking step

proprietary blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Type

Other

Abcam user community

Verified customer

Submitted Sep 12 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Lung)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

22 µg

Specification

Lung

Blocking step

Odyssey TBS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

Pete Serbedzija

Verified customer

Submitted Jul 28 2016

1-10 of 16 Abreviews or Q&A

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Recombinant Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315)

Overview

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References

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Customer reviews and Q&As

Western blot abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Western blot abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

ELISA abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Western blot abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]