Recombinant Anti-STAT4 antibody [8/Stat4] (ab280213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [8/Stat4] to STAT4
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-STAT4 antibody [8/Stat4]
See all STAT4 primary antibodies -
Description
Mouse monoclonal [8/Stat4] to STAT4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse testis, Rat testis tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8/Stat4 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 85 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 85 kDa. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. -
Involvement in disease
Systemic lupus erythematosus 11
Rheumatoid arthritis -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 20849 Mouse
- Entrez Gene: 367264 Rat
- SwissProt: P42228 Mouse
- Unigene: 1550 Mouse
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Alternative names
- Signal transducer and activator of transcription 4 antibody
- SLEB11 antibody
- STAT4 antibody
- STAT4_HUMAN antibody
Images
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All lanes : Anti-STAT4 antibody [8/Stat4] (ab280213) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat testis tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 6 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 85 kDaBlocking and Diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver, brain (PMID: 8007943)
Exposure time: 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab280213 has not yet been referenced specifically in any publications.