Overview

  • Product name

    Anti-STAT4 antibody [EP1900Y]
    See all STAT4 primary antibodies
  • Description

    Rabbit monoclonal [EP1900Y] to STAT4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human STAT4 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14765

  • Positive control

    • WB: Daudi, K562 and Hela cell lysates. IHC-P: Human tonsil tissue sections.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab68156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 30, 86 kDa (predicted molecular weight: 86 kDa).
IP 1/20.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • Involvement in disease

    Systemic lupus erythematosus 11
    Rheumatoid arthritis
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Images

  • All lanes : Anti-STAT4 antibody [EP1900Y] (ab68156) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

  • ab68156, at 1/100 dilution, staining STAT4 in paraffin embedded human tonsil tissue sections by Immunohistochemistry

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Xu YP  et al. Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy. J Clin Invest 130:4316-4331 (2019). Read more (PubMed: 31310587) »
  • Sun W  et al. 'Psoriasis 1' reduces psoriasis-like skin inflammation by inhibiting the VDR-mediated nuclear NF-?B and STAT signaling pathways. Mol Med Rep 18:2733-2743 (2018). Read more (PubMed: 30015892) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human Vgamma9 Vdelta2 T cells)
Total protein in input
3e+006 cells
Immuno-precipitation step
Other - Dynabeads (Protein G)
Specification
human Vgamma9 Vdelta2 T cells

Abcam user community

Verified customer

Submitted Nov 07 2016

Application
Flow Cytometry
Sample
Human Cell (Purified human Vgamma9 Vdelta2 T-cells)
Permeabilization
Yes - Foxp3 Staining Buffer Set
Gating Strategy
CD3 and V delta 2 positive population
Specification
Purified human Vgamma9 Vdelta2 T-cells
Fixation
Foxp3 Staining Buffer Set

Abcam user community

Verified customer

Submitted Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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