Product nameSTAT4 overexpression 293T lysate (whole cell)
DescriptionSTAT4 overexpression lysate
ab94123 is a 293T cell transfected lysate in which Human STAT4 has been transiently over-expressed using a pCMV-STAT4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundDisease: Systemic lupus erythematosus 11 Rheumatoid arthritis Function: Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. PTM: Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Our Abpromise guarantee covers the use of ab94123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94123 at 15µg/lane on an SDS-PAGE gel.
All lanes : STAT4 antibody at 1/500 dilution
Lane 1 :
STAT4 overexpression 293T lysate (whole cell) (ab94123)
Lane 2 : 293T non-transfected cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94123 has not yet been referenced specifically in any publications.