STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)

ab126428 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT4 (Tyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines. By determining STAT4 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

In the STAT4 (Tyr693) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT4 (Tyr693) or Anti-STAT4 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-Mouse IgG or HRP-conjugated anti-rabbit IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Signal Transduction
• Signaling Pathway
• Nuclear Signaling
• STATs

• Epigenetics and Nuclear Signaling
• Transcription
• Other factors

• Epigenetics and Nuclear Signaling
• Nuclear Signaling Pathways
• STATs

• Epigenetics and Nuclear Signaling
• Transcription
• Transcription Factors

• Cancer
• Signal transduction
• Nuclear signaling
• STAT family

• Kits/ Lysates/ Other
• Kits
• ELISA Kits
• In Cell ELISA Kits
• Phosphoprotein ICE kits