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Signal Transduction Signaling Pathway Nuclear Signaling STATs
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STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)

  • Datasheet
  • SDS
  • Protocol Booklet
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In cell ELISA - STAT4 (Tyr693) In-Cell ELISA kit (ab126428)
  • Western blot - STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)

Key features and details

  • Assay type: Cell-based (qualitative)
  • Detection method: Colorimetric
  • Sample type: Adherent cells
  • Reacts with: Mouse, Human

Overview

  • Product name

    STAT4 (Tyr693) In-Cell ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells
  • Assay type

    Cell-based (qualitative)
  • Assay time

    5h 10m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Human
  • Product overview

    ab126428 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT4 (Tyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines. By determining STAT4 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

    In the STAT4 (Tyr693) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT4 (Tyr693) or Anti-STAT4 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-Mouse IgG or HRP-conjugated anti-rabbit IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    Blocking Buffer Concentrate (5X) 1 x 20ml
    Fixing Solution 1 x 30ml
    HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl
    Anti-Rabbit IgG Concentrate (Item I-1) 1 x 10µl
    Uncoated 96-well Microplate 1 unit
    Mouse Anti-STAT4 Concentrate (Item H) 1 x 7µl
    Quenching Buffer Concentrate (30x) 1 x 2ml
    Rabbit Anti-Phospho-STAT4 (Tyr693) Concentrate (Item G) 1 x 10µl
    Stop Solution 1 x 14ml
    TMB One-Step Substrate Reagent 1 x 12ml
    Wash Buffer A Concentrate (20X) 1 x 30ml
    Wash Buffer B Concentrate (20X) 1 x 30ml
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • In Cell ELISA Kits
    • Phosphoprotein ICE kits
  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • Involvement in disease

    Systemic lupus erythematosus 11
    Rheumatoid arthritis
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Target information above from: UniProt accession Q14765 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Signal transducer and activator of transcription 4
    • SLEB11
    • STAT4
    • STAT4_HUMAN
  • Database links

    • Entrez Gene: 6775 Human
    • Entrez Gene: 20849 Mouse
    • Omim: 600558 Human
    • SwissProt: Q14765 Human
    • SwissProt: P42228 Mouse
    • Unigene: 735572 Human
    • Unigene: 80642 Human
    • Unigene: 1550 Mouse

    Images

    • In cell ELISA - STAT4 (Tyr693) In-Cell ELISA kit (ab126428)
      In cell ELISA - STAT4 (Tyr693) In-Cell ELISA kit (ab126428)

      A431 cells were stimulated by different concentrations of recombinant human EGF for 10 minutes at 37°C

    • Western blot - STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)
      Western blot - STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)
      Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-STAT4 (Tyr693) and STAT4 antibodies were used in both detection assays.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab126428? Please let us know so that we can cite the reference in this datasheet.

    ab126428 has not yet been referenced specifically in any publications.

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