STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)
- Datasheet
- References
- Protocols
Overview
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Product nameSTAT4 (Tyr693) In-Cell ELISA Kit
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Detection methodColorimetric
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Sample typeAdherent cells
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Assay typeCell-based (qualitative)
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Assay time5h 10m
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse, Human
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Product overview
ab126428 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT4 (Tyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines. By determining STAT4 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the STAT4 (Tyr693) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT4 (Tyr693) or Anti-STAT4 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-Mouse IgG or HRP-conjugated anti-rabbit IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applicationsSuitable for: In-Cell ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests Blocking Buffer Concentrate (5X) 1 x 20ml Fixing Solution 1 x 30ml HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl Anti-Rabbit IgG Concentrate (Item I-1) 1 x 10µl Uncoated 96-well Microplate 1 unit Mouse Anti-STAT4 Concentrate (Item H) 1 x 7µl Quenching Buffer Concentrate (30x) 1 x 2ml Rabbit Anti-Phospho-STAT4 (Tyr693) Concentrate (Item G) 1 x 10µl Stop Solution 1 x 14ml TMB One-Step Substrate Reagent 1 x 12ml Wash Buffer A Concentrate (20X) 1 x 30ml Wash Buffer B Concentrate (20X) 1 x 30ml -
Research areas
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FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
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Involvement in diseaseSystemic lupus erythematosus 11
Rheumatoid arthritis -
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
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Alternative names
- Signal transducer and activator of transcription 4
- SLEB11
- STAT4
- STAT4_HUMAN
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Database links
- Entrez Gene: 6775 Human
- Entrez Gene: 20849 Mouse
- Omim: 600558 Human
- SwissProt: Q14765 Human
- SwissProt: P42228 Mouse
- Unigene: 735572 Human
- Unigene: 80642 Human
- Unigene: 1550 Mouse
Applications
Our Abpromise guarantee covers the use of ab126428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| In-Cell ELISA | Use at an assay dependent concentration. |
Images
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In cell ELISA - STAT4 (Tyr693) In-Cell ELISA kit (ab126428)A431 cells were stimulated by different concentrations of recombinant human EGF for 10 minutes at 37°C
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Western blot - STAT4 (Tyr693) In-Cell ELISA Kit (ab126428)Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-STAT4 (Tyr693) and STAT4 antibodies were used in both detection assays.
Protocols
References
ab126428 has not yet been referenced specifically in any publications.
