For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    stat5-ab-py694699-elisa-kit-ab176656.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Signaling Pathway Nuclear Signaling STATs
Share by email
SimpleStep

STAT5 A/B pY694/699 ELISA Kit (ab176656)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Other - STAT5 A/B pY694/699 ELISA Kit (ab176656)
  • Typical cell lysate dilution series
  • Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment.
  • Cell line analysis for Total STAT5 A/B

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5 µg/ml
  • Range: 10 µg/ml - 1000 µg/ml
  • Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Homogenate
  • Detection method: Colorimetric
  • Assay type: Semi-quantitative
  • Reacts with: Human

You may also be interested in

ELISA
Product image
STAT3 pY705 ELISA Kit (ab176654)
ELISA
Product image
STAT 5 A/B ELISA Kit (ab205714)
Assay
Product image
STAT (STAT1alpha, STAT3, STAT5A, STAT5B) Transcription Factor Assay Kit (Colorimetric) (ab207228)

View more associated products

Overview

  • Product name

    STAT5 A/B pY694/699 ELISA Kit
    See all STAT5 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    A431 extract 6 2.8%
    Inter-assay
    Sample n Mean SD CV%
    A431 extract 3 4%
  • Sample type

    Cell culture extracts, Adherent cells, Suspension cells, Tissue Homogenate
  • Assay type

    Semi-quantitative
  • Sensitivity

    < 5 µg/ml
  • Range

    10 µg/ml - 1000 µg/ml
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Product overview

    Abcam’s STAT5 A/B (pY694/699) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of STAT5 A/B (pY694/699) protein in Human cells.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm

  • Notes

    Abcam’s STAT5 A/B (pY694/699) SimpleStep ELISA kit (ab176656) has been re-developed with new detector antibody.  From February 18, 2016 the kit will be delivered with a new detector total STAT5 antibody. This change was necessary because of a decrease in the performance of the polyclonal STAT5 antibody that was previously used for detection in recent production lots. We have identified a new antibody to use in the SimpleStep ELISA platform that provides higher performance.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT 1 x 15ml
    50X Cell Extraction Enhancer Solution 1 x 1ml
    5X Cell Extraction Buffer PTR 1 x 12ml
    Lyophilized STAT5 A/B Control Lysate 1 vial
    Plate Seal 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    STAT5 A/B (pY694/699) Capture Antibody 1 x 3ml
    STAT5 A/B (pY694/699) Detector Antibody 1 x 3ml
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Phosphoprotein ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
  • Function

    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Target information above from: UniProt accession P42229 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • MGF
    • Signal transducer and activator of transcription 5A
    • Signal Transducer and Activator of Transcription 5B
    • STA5A_HUMAN
    • STAT 5A
    • STAT 5B
    • STAT5
    • STAT5A
    • STAT5B
    • Transcription factor STAT5A
    • Transcription factor STAT5B
    see all
  • Database links

    • Entrez Gene: 6777 Human
    • Entrez Gene: 6776 Human
    • Entrez Gene: 20851 Mouse
    • Entrez Gene: 20850 Mouse
    • Entrez Gene: 25126 Rat
    • Entrez Gene: 24918 Rat
    • Omim: 601511 Human
    • Omim: 604260 Human
    • SwissProt: P51692 Human
    • SwissProt: P42229 Human
    • SwissProt: P42232 Mouse
    • SwissProt: P42230 Mouse
    • SwissProt: P52632 Rat
    • SwissProt: Q62771 Rat
    • Unigene: 437058 Human
    • Unigene: 277403 Mouse
    • Unigene: 154399 Rat
    see all

Associated products

    Images

    • Other - STAT5 A/B pY694/699 ELISA Kit (ab176656)
      Other - STAT5 A/B pY694/699 ELISA Kit (ab176656)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Typical cell lysate dilution series
      Typical cell lysate dilution series

      Example of a typical STAT5 A/B (pY694/699) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.

    • Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment.
      Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment.

      A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT5 A/B (pY694/699) are plotted and compared against Total STAT5 A/B levels.

    • Cell line analysis for Total STAT5 A/B
      Cell line analysis for Total STAT5 A/B

      Cell line analysis for Total STAT5 A/B from 100 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab176656? Please let us know so that we can cite the reference in this datasheet.

    ab176656 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab176656.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.