Overview

  • Product name

    Anti-STAT5 antibody [9F7]
    See all STAT5 primary antibodies
  • Description

    Mouse monoclonal [9F7] to STAT5
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human STAT5.
    Database link: P42229

  • Positive control

    • WB: STAT5-transfected HEK-293T cell lysate; HepG2, HeLa, SVT2, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts. IHC-P: HUman testis tissue. ICC/IF: COS7 cells. Flow Cyt: HeLa and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100.
ICC/IF 1/100.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • Signal Transducer and Activator of Transcription 5B antibody
    • STA5A_HUMAN antibody
    • STAT 5A antibody
    • STAT 5B antibody
    • STAT5 antibody
    • STAT5A antibody
    • STAT5B antibody
    • Transcription factor STAT5A antibody
    • Transcription factor STAT5B antibody
    see all

Images

  • All lanes : Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution

    Lane 1 : Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : STAT5-transfected HEK-293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.
  • All lanes : Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 (human lung carcinoma cell line) cell extract
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 7 : MDCK (canine kidney cell line) cell extract
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.
  • Formalin-fixed, paraffin-embedded human testis tissue stained for STAT5 with ab230670 at 10 μg/ml in immunohistochemical analysis.

  • COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with STAT5 and stained for STAT5 (green) using ab230670 at 1/100 dilution. 

     

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).

  • Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).

References

ab230670 has not yet been referenced specifically in any publications.

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