Anti-STAT5 antibody (ab16276)
Key features and details
- Rabbit polyclonal to STAT5
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-STAT5 antibody
See all STAT5 primary antibodies -
Description
Rabbit polyclonal to STAT5 -
Host species
Rabbit -
Specificity
This antibody does not cross react with any other STATs or related proteins -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Cow
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Immunogen
Synthetic peptide:
ATIISEQQAKSLLKNENTR
, corresponding to amino acids 371-389 of Human STAT5. -
Positive control
- CTLL cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
PBS is Magnesium and Calcium free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Detects bands of approximately 92 kDa and 85 kDa representing STAT5a and STAT5b respectively from CTLL cell lysate.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Detects bands of approximately 92 kDa and 85 kDa representing STAT5a and STAT5b respectively from CTLL cell lysate. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 443419 Sheep
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: Q95115 Cow
- SwissProt: Q9TUM3 Cow
- SwissProt: P42229 Human
see all -
Alternative names
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
Images
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Western blot - Anti-STAT5 antibody (ab16276)Anti-STAT5 antibody (ab16276) + SK-BR-3 Human adenocarcinoma cell line
Secondary
HRP conjugated goat anti-rabbit
Predicted band size: 90 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody (ab16276)ICC/IF image of ab71498 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab16276 has been referenced in 15 publications.
- Li R et al. Identification and In Vitro Functional Verification of Two Novel Mutations of GHR Gene in the Chinese Children with Laron Syndrome. Front Endocrinol (Lausanne) 12:605736 (2021). PubMed: 33912130
- Ma F et al. Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway. Theranostics 10:7730-7746 (2020). PubMed: 32685016
- Lyu X et al. Acute sleep deprivation leads to growth hormone (GH) resistance in rats. Gen Comp Endocrinol 296:113545 (2020). PubMed: 32622934
- Sun Z et al. NHE1 Mediates 5-Fu Resistance in Gastric Cancer via STAT3 Signaling Pathway. Onco Targets Ther 13:8521-8532 (2020). PubMed: 32904684
- Chen Z et al. Long non-coding RNA XIST promotes the development of esophageal cancer by sponging miR-494 to regulate CDK6 expression. Biomed Pharmacother 109:2228-2236 (2019). PubMed: 30551480
