Anti-STAT5 antibody (ab16276)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-STAT5 antibody
See all STAT5 primary antibodies -
DescriptionRabbit polyclonal to STAT5
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Host speciesRabbit
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SpecificityThis antibody does not cross react with any other STATs or related proteins
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow
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Immunogen
Synthetic peptide:
ATIISEQQAKSLLKNENTR
, corresponding to amino acids 371-389 of Human STAT5. -
Positive control
- CTLL cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
PBS is Magnesium and Calcium free -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab16276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Detects bands of approximately 92 kDa and 85 kDa representing STAT5a and STAT5b respectively from CTLL cell lysate. |
Target
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FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
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Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
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Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Entrez Gene: 24918 Rat
- Entrez Gene: 25126 Rat
- Entrez Gene: 443419 Sheep
- Omim: 601511 Human
see all -
Alternative names
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
Images
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Western blot - Anti-STAT5 antibody (ab16276)Anti-STAT5 antibody (ab16276) + SK-BR-3 Human adenocarcinoma cell line
Secondary
HRP conjugated goat anti-rabbit
Predicted band size: 90 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-STAT5 antibody (ab16276)ICC/IF image of ab71498 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Yang L et al. Enhancing NK cell-mediated cytotoxicity to cisplatin-resistant lung cancer cells via MEK/Erk signaling inhibition. Sci Rep 7:7958 (2017). WB ; Human . Read more (PubMed: 28801607) »
- Elliott S et al. Lack of expression and function of erythropoietin receptors in the kidney. Nephrol Dial Transplant : (2011). WB ; Human . Read more (PubMed: 22167585) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"