Key features and details
- Rabbit polyclonal to STAT5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STAT5 antibody
See all STAT5 primary antibodies
DescriptionRabbit polyclonal to STAT5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Cow
Synthetic peptide corresponding to Human STAT5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: MCF7; Jurkat.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).|
FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
All lanes : Anti-STAT5 antibody (ab88246) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Additional bands at: 140 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
ab88246 has not yet been referenced specifically in any publications.