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To whom this may concern, I am working with a b32364 and having problems getting any detectable protein using western blotting from either human primary macrophages or murine bone derived macrophages. I would like to use a positive control for the blot and was hoping that you would be able to recommend a product for both murine and human detection.
Asked on Nov 22 2011
Thank you for contacting us. For a positive control for human cells, I recommend the nuclear lysate of EGF-treated A431 cells which we have in our catalogue as ab14635: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14635). For mouse cells, I recommend a lysate of 3T3 cells treated with leukemia inhibitory facotr (LIF), as seen in the western blot shown on the datasheet for ab13539, but we do not have this lysate as a product. There may be other treatments of mouse cells that will induce the phosphorylation, but we are not aware of them. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 22 2011