Recombinant Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E208] to STAT5 (phospho Y694) - BSA and Azide free
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free
See all STAT5 primary antibodies -
Description
Rabbit monoclonal [E208] to STAT5 (phospho Y694) - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody only detects Stat5 phosphorylated on Tyrosine 694. -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.
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General notes
ab219607 is the carrier-free version of ab32364.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E208 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-STAT5 (phospho Y694) antibody [E208] (ab310959)
- Alexa Fluor® 647 Anti-STAT5 (phospho Y694) antibody [E208] (ab311076)
- Alexa Fluor® 594 Anti-STAT5 (phospho Y694) antibody [E208] (ab311665)
- Alexa Fluor® 568 Anti-STAT5 (phospho Y694) antibody [E208] (ab312939)
- Alexa Fluor® 555 Anti-STAT5 (phospho Y694) antibody [E208] (ab313150)
- Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: P42229 Human
- SwissProt: P51692 Human
see all -
Alternative names
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
Images
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Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32364).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32364).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab219607 has not yet been referenced specifically in any publications.