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    stat5a--stat5b-antibody-epr16671-40-ab194898.pdf

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RecombinantRabMAb

Recombinant Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

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  • Certificate of Compliance
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Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Flow Cytometry (Intracellular) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
  • Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16671-40] to STAT5a + STAT5b
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-STAT5a + STAT5b antibody [EPR16671-40]
  • Description

    Rabbit monoclonal [EPR16671-40] to STAT5a + STAT5b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human STAT5A full length recombinant protein; K562, Jurkat and Daudi cell lysates; Human fetal heart and kidney lysates; mouse heart, kidney, spleen lysates; rat brain, heart, spleen lysates; RAW 264.7, PC12, NIH/3T3 whole cell lysates. IHC-P: Human tonsils, mouse spleen and rat spleen tissue. IF, Flow, IP: K562 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16671-40
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Associated products

  • Alternative Versions

    • Anti-STAT5a + STAT5b antibody [EPR16671-40] - BSA and Azide free (ab215367)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (2)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
1/2000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
ICC/IF
1/250.
IP
1/100.
Notes
Flow Cyt (Intra)
1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
ICC/IF
1/250.
IP
1/100.

Target

  • Cellular localization

    STAT5a: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. STAT5b: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Database links

    • Entrez Gene: 6776 Human
    • Entrez Gene: 6777 Human
    • Entrez Gene: 20850 Mouse
    • Entrez Gene: 20851 Mouse
    • Entrez Gene: 24918 Rat
    • Entrez Gene: 25126 Rat
    • Omim: 601511 Human
    • Omim: 604260 Human
    • SwissProt: P42229 Human
    • SwissProt: P51692 Human
    • SwissProt: P42230 Mouse
    • SwissProt: P42232 Mouse
    • SwissProt: P52632 Rat
    • SwissProt: Q62771 Rat
    • Unigene: 437058 Human
    • Unigene: 595276 Human
    • Unigene: 277403 Mouse
    • Unigene: 34064 Mouse
    • Unigene: 154399 Rat
    • Unigene: 54486 Rat
    see all

Images

  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    All lanes : Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898) at 1/20000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 3 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling STAT5a + STAT5b with ab194898 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on K562 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor® 594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab194898 at 1/250 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    All lanes : Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898) at 1/2000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human fetal kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 91 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898) at 1/1000 dilution + Human STAT5A full length recombinant protein at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91 kDa



    Recombinant human C-terminal Myc/DDK tagged full length STAT5A from a commercial source.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Western blot - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    All lanes : Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898) at 1/2000 dilution

    Lane 1 : mouse heart lysates
    Lane 2 : mouse kidney lysates
    Lane 3 : mouse spleen lysates
    Lane 4 : rat brain lysates
    Lane 5 : rat heart lysates
    Lane 6 : rat spleen lysates
    Lane 7 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 8 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 9 : NIH3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling STAT5a + STAT5b using ab194898 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counter stained with Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194898.
    Nuclear and weak cytoplasmic staining on lymphocytes of human tonsil is observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling STAT5a + STAT5b using ab194898 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counter stained with Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194898.
    Nuclear and weak cytoplasmic staining on lymphocytes of mouse spleen is observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling STAT5a + STAT5b using ab194898 at 1/2000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counter stained with Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194898.
    Nuclear and weak cytoplasmic staining on lymphocytes of rat spleen is observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Flow Cytometry (Intracellular) - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling STAT5a +STAT5b with ab194898 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    STAT5a and STAT5b were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) cells with ab194898 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab194898 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, was used as secondary antibody at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    STAT5a + STAT5b were co-immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) cells with ab194898 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab32043 (Rabbit monoclonal [E289] to STAT5a) at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, was used as secondary antibody at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Immunoprecipitation - Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

    STAT5a + STAT5b were co-immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) cells with ab194898 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab178941 (Rabbit monoclonal [EPR16671] to STAT5b) at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, was used as secondary antibody at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)
    Anti-STAT5a + STAT5b antibody [EPR16671-40] (ab194898)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (8)

Publishing research using ab194898? Please let us know so that we can cite the reference in this datasheet.

ab194898 has been referenced in 8 publications.

  • Høyer KL  et al. The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals. Physiol Rep 8:e14373 (2020). PubMed: 32073221
  • Zhang Y Effect of iron chelation therapy on EPO-STAT5 signalling pathway and EPO resistance in iron-overloaded low-risk myelodysplastic syndrome patients. Hematology 25:1-10 (2020). PubMed: 31838956
  • Aikawa T  et al. Quizartinib, a selective FLT3 inhibitor, maintains antileukemic activity in preclinical models of RAS-mediated midostaurin-resistant acute myeloid leukemia cells. Oncotarget 11:943-955 (2020). PubMed: 32215183
  • Shan S  et al. Therapeutic treatment of a novel selective JAK3/JAK1/TBK1 inhibitor, CS12192, in rat and mouse models of rheumatoid arthritis. Int Immunopharmacol 77:105914 (2019). PubMed: 31634789
  • Quagliarini F  et al. Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet. Mol Cell 76:531-545.e5 (2019). PubMed: 31706703
  • Ding L  et al. Deletion of Cdkn1b in ACI rats leads to increased proliferation and pregnancy-associated changes in the mammary gland due to perturbed systemic endocrine environment. PLoS Genet 15:e1008002 (2019). PubMed: 30893315
  • You YH  et al. MicroRNA-153 promotes brain-derived neurotrophic factor and hippocampal neuron proliferation to alleviate autism symptoms through inhibition of JAK-STAT pathway by LEPR. Biosci Rep 39:N/A (2019). PubMed: 30975733
  • Mitchell J  et al. Validation of a Preclinical Model of Diethylnitrosamine-Induced Hepatic Neoplasia in Yucatan Miniature Pigs. Oncology 91:90-100 (2016). PubMed: 27305144

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT5a + STAT5b antibody [EPR16671-40]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More

Abcam user community

Verified customer

Submitted Jul 31 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT5a + STAT5b antibody [EPR16671-40]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More

Abcam user community

Verified customer

Submitted Jul 31 2019

Western blot abreview for Anti-STAT5a + STAT5b antibody [EPR16671-40]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (human sceletal muscle)
Gel Running Conditions
Reduced Denaturing (WES simple western)
Loading amount
1 µg
Specification
human sceletal muscle
Blocking step
antibody dilution from protein simple as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Read More

Helle Zibrandtsen

Verified customer

Submitted Mar 07 2018

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