Overview

  • Product name
  • Description
    Goat polyclonal to STAT5a
  • Host species
    Goat
  • Specificity
    ab223682 is not expected to cross-react with STAT5B.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human STAT5a aa 681-692 (internal sequence) (Cysteine residue). NP_003143.2.
    Sequence:

    C-KYYTPVLAKAVD


    Database link: P42229

  • Positive control
    • WB: K562 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab223682 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 90 kDa).

Target

Images

  • Anti-STAT5a antibody (ab223682) at 0.1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 90 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223682 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223682.
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