Key features and details
- Goat polyclonal to STAT5a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STAT5a antibody
See all STAT5a primary antibodies
DescriptionGoat polyclonal to STAT5a
Specificityab223682 is not expected to cross-react with STAT5B.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
- WB: K562 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223682 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 90 kDa).
1 hour primary incubation is recommended for this product.
FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
Anti-STAT5a antibody (ab223682) at 0.1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223682 has not yet been referenced specifically in any publications.