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Rabbit polyclonal to STAT5a - C-terminal
Predicted to work with:
Mouse, Rat, Cow
Recombinant fragment corresponding to a region within C terminal amino acids 564-786 of Human STAT5a (UniProt P42229).
293T, A431, HeLa and HepG2 whole cell lysates; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 90 kDa.
1/100 - 1/1000.
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Information by UniProt
Mammary gland factor antibody
Signal transducer and activator of transcription 5A antibody
Western blot - Anti-STAT5a antibody - C-terminal (ab155036)
All lanes : Anti-STAT5a antibody - C-terminal (ab155036) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : HeLa whole cell lysate Lane 4 : HepG2 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 90 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody - C-terminal (ab155036)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STAT5a with ab155036 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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