Product nameAnti-STAT5a antibody - C-terminal
See all STAT5a primary antibodies
DescriptionRabbit polyclonal to STAT5a - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to a region within C terminal amino acids 564-786 of Human STAT5a (UniProt P42229).
- 293T, A431, HeLa and HepG2 whole cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 90 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
All lanes : Anti-STAT5a antibody - C-terminal (ab155036) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
7.5% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STAT5a with ab155036 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
ab155036 has not yet been referenced specifically in any publications.