Anti-STAT5a antibody - C-terminal (ab155036)

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Overview

  • Product name
    Anti-STAT5a antibody - C-terminal
    See all STAT5a primary antibodies
  • Description
    Rabbit polyclonal to STAT5a - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within C terminal amino acids 564-786 of Human STAT5a (UniProt P42229).

  • Positive control
    • 293T, A431, HeLa and HepG2 whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 90 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Images

  • Western blot - Anti-STAT5a antibody - C-terminal (ab155036)
    Western blot - Anti-STAT5a antibody - C-terminal (ab155036)
    All lanes : Anti-STAT5a antibody - C-terminal (ab155036) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 90 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody - C-terminal (ab155036)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody - C-terminal (ab155036)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STAT5a with ab155036 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab155036 has not yet been referenced specifically in any publications.

Publishing research using ab155036? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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ChIP abreview for Anti-STAT5a antibody - C-terminal

 Excellent
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
abreview image
Application
ChIP
Sample
Human Cell lysate - whole cell (Human Breast Cancer -- T47D)
Negative control
non-specific rabbit IgG
Specification
Human Breast Cancer -- T47D
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 540 ul 37% Formaldehyde into 2
Positive control
+Prolactin (100ng) for 45 minutes qRT-PCR looking at STAT5A recruitment to -700kb of transcriptional start site of known STAT5A gene (CISH)
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Verified customer

Submitted Jan 30 2019

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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