Anti-STAT5a antibody - C-terminal (ab155036)

Product name

Anti-STAT5a antibody - C-terminal
See all STAT5a primary antibodies
Description

Rabbit polyclonal to STAT5a - C-terminal
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
Immunogen

Recombinant fragment corresponding to a region within C terminal amino acids 564-786 of Human STAT5a (UniProt P42229).
Positive control
- 293T, A431, HeLa and HepG2 whole cell lysates; HeLa cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab155036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/3000. Predicted molecular weight: 90 kDa.
ICC/IF		1/100 - 1/1000.

Function

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications

Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localization

Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Target information above from: UniProt accession P42229 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 282375 Cow
- Entrez Gene: 6776 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- SwissProt: Q95115 Cow
- SwissProt: P42229 Human
- SwissProt: P42230 Mouse
- SwissProt: Q62771 Rat
- Unigene: 437058 Human
- Unigene: 277403 Mouse
- Unigene: 154399 Rat
see all
Alternative names
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- STA5A_HUMAN antibody
- STAT 5 antibody
- STAT 5A antibody
- STAT5 antibody
- STAT5A antibody
see all

Western blot - Anti-STAT5a antibody - C-terminal (ab155036)

All lanes : Anti-STAT5a antibody - C-terminal (ab155036) at 1/1000 dilution

Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate

Lysates/proteins at 30 µg per lane.

Predicted band size: 90 kDa

7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody - C-terminal (ab155036)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STAT5a with ab155036 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

ab155036 has not yet been referenced specifically in any publications.

Publishing research using ab155036? Please let us know so that we can cite the reference in this datasheet.

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