Anti-STAT5a antibody - C-terminal (ab155036)
Key features and details
- Rabbit polyclonal to STAT5a - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT5a antibody - C-terminal
See all STAT5a primary antibodies -
Description
Rabbit polyclonal to STAT5a - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to a region within C terminal amino acids 564-786 of Human STAT5a (UniProt P42229).
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Positive control
- 293T, A431, HeLa and HepG2 whole cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 90 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 90 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 282375 Cow
- Entrez Gene: 6776 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- SwissProt: Q95115 Cow
- SwissProt: P42229 Human
- SwissProt: P42230 Mouse
see all -
Alternative names
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
see all
Images
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Western blot - Anti-STAT5a antibody - C-terminal (ab155036)All lanes : Anti-STAT5a antibody - C-terminal (ab155036) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 90 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-STAT5a antibody - C-terminal (ab155036)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STAT5a with ab155036 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
References (0)
ab155036 has not yet been referenced specifically in any publications.
