ChIP abreview for Anti-STAT5a antibody - C-terminal

Excellent
Abreviews
Application
ChIP
Sample
Human Cell lysate - whole cell (Human Breast Cancer -- T47D)
Negative control
non-specific rabbit IgG
Specification
Human Breast Cancer -- T47D
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 540 ul 37% Formaldehyde into 2
Positive control
+Prolactin (100ng) for 45 minutes qRT-PCR looking at STAT5A recruitment to -700kb of transcriptional start site of known STAT5A gene (CISH)

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
0.01 µl/µg chromatin

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Submitted Jan 30 2019

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