Overview

  • Product name
    Anti-STAT5a antibody - C-terminal
    See all STAT5a primary antibodies
  • Description
    Goat polyclonal to STAT5a - C-terminal
  • Host species
    Goat
  • Specificity
    ab223681 is not expected to cross-react with STAT5b.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human STAT5a aa 776-790 (C terminal) (Cysteine residue). NP_003143.2.
    Sequence:

    C-DSRLSPPAGLFTSAR


    Database link: P42229

  • Positive control
    • WB: K562, MOLT-4 and NIH/3T3 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab223681 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 90 kDa).

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Images

  • All lanes : Anti-STAT5a antibody - C-terminal (ab223681) at 0.3 µg/ml

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate in RIPA buffer
    Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate in RIPA buffer
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 90 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223681 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223681.
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