Key features and details
- Rabbit polyclonal to STAT5a - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STAT5a antibody - C-terminal
See all STAT5a primary antibodies
DescriptionRabbit polyclonal to STAT5a - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
Synthetic peptide within Human STAT5a aa 750 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P42229
- WB: K562 cell lysate. IHC-P: Human breast carcinoma tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab231489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/25. Predicted molecular weight: 91 kDa.
Primary antibody incubation for 1 hour at room temperature.
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary antibody incubation for 10 minutes at room temperature.
FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for STAT5a using ab231489 at 1/200 dilution in immunohistochemical analysis.
Anti-STAT5a antibody - C-terminal (ab231489) at 1/25 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 91 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231489 has not yet been referenced specifically in any publications.