Product nameSTAT5a overexpression 293T lysate (whole cell)
ab94124 is a 293T cell transfected lysate in which Human STAT5a has been transiently over-expressed using a pCMV-STAT5a plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Our Abpromise guarantee covers the use of ab94124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94124 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-STAT5a antibody (ab57823) at 1/500 dilution
Lane 1 :
STAT5a overexpression 293T lysate (whole cell) (ab94124)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94124 has not yet been referenced specifically in any publications.