Key features and details
- Rabbit polyclonal to STAT5a (phospho S725) + STAT5b (phospho S730)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody
DescriptionRabbit polyclonal to STAT5a (phospho S725) + STAT5b (phospho S730)
Specificityab194518 detects endogenous levels of STAT5a + STAT5b only when phosphorylated at serine 725 / serine 730.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human STAT5a (phospho S725) conjugated to keyhole limpet haemocyanin. (A-P-S(p)-P-V)
Database link: P42230
Synthetic peptide corresponding to Human STAT5b (phospho S730) conjugated to keyhole limpet haemocyanin. (A-P-S(p)-P-V)
Database link: P42232
- EGF treated Jurkat cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab194518 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab194518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 91, 90 kDa.|
Cellular localizationSTAT5a: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. STAT5b: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
ab194518 has been referenced in 1 publication.
- You YH et al. MicroRNA-153 promotes brain-derived neurotrophic factor and hippocampal neuron proliferation to alleviate autism symptoms through inhibition of JAK-STAT pathway by LEPR. Biosci Rep 39:N/A (2019). PubMed: 30975733