Recombinant
RabMAb

Recombinant Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)

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Overview

  • Product name

    Anti-STAT5a (phospho S726) antibody [EPR1914(2)]
    See all STAT5a primary antibodies

  • Description

    Rabbit monoclonal [EPR1914(2)] to STAT5a (phospho S726)

  • Host species

    Rabbit

  • Specificity

    ab128896 only detects STAT5a phosphorylated at Serine 726

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: FuncS,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Ser726 of Human STAT5a (UniProt P42229).

  • Positive control

    • SH-SY5Y cell lysates treated with Okadaic acid and Calyculin A.

  • General notes

    A trial size is available to purchase for this antibody.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab128896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
  • Application notes
    Is unsuitable for FuncS,ICC or IHC-P.

    • Target

    • Function

      Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

    • Sequence similarities

      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.

    • Post-translational
      modifications

      Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.

    • Cellular localization

      Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Mammary gland factor antibody
      • MGF antibody
      • Signal transducer and activator of transcription 5A antibody
      • STA5A_HUMAN antibody
      • STAT 5 antibody
      • STAT 5A antibody
      • STAT5 antibody
      • STAT5A antibody
      see all

    Images

    • Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
      Dot Blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)

      Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: STAT5a (pS726) phospho peptide

      Lane 2 sample: STAT5a non-phospho peptide

      Exposure time: 10 seconds

    • Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
      Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
      All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/5000 dilution

      Lane 1 : SH-SY5Y (human neuroblastoma) treated with Okadaic acid and Calyculin A whole cell lysates
      Lane 2 : Untreated SH-SY5Y (human neuroblastoma) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Predicted band size: 90 kDa
      Observed band size: 90 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
      Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896)
      All lanes : Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (ab128896) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysates: untreated
      Lane 2 : SH-SY5Y cell lysates: treated with Okadaic acid and Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 90 kDa
      Observed band size: 90 kDa

    References

    ab128896 has not yet been referenced specifically in any publications.

    Publishing research using ab128896? Please let us know so that we can cite the reference in this datasheet.

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