Key features and details
- Rabbit polyclonal to STAT5a (phospho S780)
- Suitable for: ICC/IF, WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-STAT5a (phospho S780) antibody
See all STAT5a primary antibodies
DescriptionRabbit polyclonal to STAT5a (phospho S780)
Specificityab30649 detects endogenous levels of STAT5A only when phosphorylated at Serine 780.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human STAT5a. Synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of Serine 780.
Database link: P42229
- IHC: human breast carcinoma. WB: Jurkat treated with 125 ng/mL PMA for 30 minutes lysate. 293T treated with 125 ng/mL PMA for 30 minutes lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab30649 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Our Abpromise guarantee covers the use of ab30649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
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All lanes : Anti-STAT5a (phospho S780) antibody (ab30649) at 1/1000 dilution
Lane 1 : Jurkat untreated lysate
Lane 2 : Jurkat treated with 125 ng/mL PMA for 30 minutes lysate
Lane 3 : 293T untreated lysate
Lane 4 : 293T treated with 125 ng/mL PMA for 30 minutes lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 91 kDa
Loading control: beta Actin.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ab30649. The picture on the right is blocked with the phospho peptide.
ICC/IF image of ab30649 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30649, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-STAT5a (phospho S780) antibody (ab30649)
Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with synthesized phosphopeptide
Predicted band size: 91 kDa
Observed band size: 91 kDa
ab30649 has not yet been referenced specifically in any publications.