Key features and details
- Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-STAT5a (phospho Y694) antibody [6.F1]
See all STAT5a primary antibodies
DescriptionMouse monoclonal [6.F1] to STAT5a (phospho Y694)
Specificityab106095 is specific for STAT5a protein phosphorylated at Y694.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
A synthetic peptide corresponding to residues surrounding Y694 of Mouse STAT5a protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notesNK92 cell lysate; Human breast tissue; 3T3 cells.
Our Abpromise guarantee covers the use of ab106095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 91 kDa.|
|IHC-P||Use a concentration of 20 - 40 µg/ml.|
|ICC/IF||1/50 - 1/1000.|
FunctionCarries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)
Lane 1 : NK92 cell lysate, untreated
Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)
All lanes : Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution
Predicted band size: 91 kDa
ab106095 at 20µg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.
ab106095 has been referenced in 1 publication.
- Datta P et al. Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells. Cytotechnology 67:223-35 (2015). PubMed: 24468831