Overview

  • Product name
    Anti-STAT5a (phospho Y694) antibody
    See all STAT5a primary antibodies
  • Description
    Rabbit polyclonal to STAT5a (phospho Y694)
  • Host species
    Rabbit
  • Specificity
    ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Tyrosine 694.

  • Positive control
    • Breast carcinoma tissue; HeLa cells treated with EGF.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue. Left: Using ab30648 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
  • All lanes : Anti-STAT5a (phospho Y694) antibody (ab30648)

    Lane 1 : HeLa cells untreated
    Lane 2 : HeLa cells treated with EGF

    Predicted band size: 91 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Wang S  et al. Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. J Hematol Oncol 11:82 (2018). Read more (PubMed: 29898759) »
  • Matsunaga K  et al. IL-1ß directly inhibits milk lipid production in lactating mammary epithelial cells concurrently with enlargement of cytoplasmic lipid droplets. Exp Cell Res 370:365-372 (2018). Read more (PubMed: 29966663) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
ChIP
Sample
Human Cell lysate - whole cell (Human Breast Cancer --T47D)
Negative control
non-specific rabbit IgG
Specification
Human Breast Cancer --T47D
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 540 ul 37% Formaldehyde into 2
Positive control
+Prolactin (100ng) for 45 minutes qRT-PCR looking at STAT5A recruitment to +5kb of transcriptional start site of known STAT5A gene (CISH)

Abcam user community

Verified customer

Submitted Nov 02 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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