The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2 µg/mg of lysate.
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
Involvement in disease
Growth hormone insensitivity with immunodeficiency
Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Immunoprecipitation of ab65743 (2µg/mg lysate) with rat liver lysate using protein G beads (lane
1), and controls using beads alone (lane 2).
Western blot analysis by another commercially available STAT5b antibody.