Overview

  • Product name

  • Description

    Rabbit polyclonal to STAT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human STAT6 (internal sequence). The exact sequence is proprietary.
    Database link: P42226

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Mouse kidney tissue; C2C12 xenograft tissue. WB: HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 94 kDa.
IP 1/500 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Images

  • Anti-STAT6 antibody (ab227497) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 94 kDa



    7.5% SDS-PAGE gel.

  • STAT6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab227497. Western blot was performed from the immunoprecipitate using ab227497. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell lysate 50 μg.

    Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.

    Lane 3: ab227497 IP in HeLa whole cell lysate.

    10% SDS-PAGE gel.

  • Paraffin-embedded mouse kidney tissue stained for STAT6 using ab227497 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded C2C12 (mouse) xenograft tissue stained for STAT6 using ab227497 at 1/500 dilution in immunohistochemical analysis.

References

ab227497 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227497.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up