Product nameAnti-STAT6 antibody
See all STAT6 primary antibodies
DescriptionRabbit polyclonal to STAT6
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human STAT6 aa 600-650. The exact sequence is proprietary. NP_003144.3
Database link: P42226
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab246008 was affinity purified using an epitope specific to STAT6 immobilized on solid support.
Our Abpromise guarantee covers the use of ab246008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 94 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for STAT6 using ab246008 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-STAT6 antibody (ab246008) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) hole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes
STAT6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab246008 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab246008 at 1 µg/ml.
Lane 1: ab246008 (Batch 1) IP in HeLa whole cell lysate.
Lane 2: ab246008 (Batch 2) IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab246008 has not yet been referenced specifically in any publications.