Product nameAnti-STAT6 antibody [E265]
See all STAT6 primary antibodies
DescriptionRabbit monoclonal [E265] to STAT6
Specificityab32108 recognises Stat-6. It does not cross react with other STAT family members
Tested applicationsSuitable for: WB, Flow Cyt, IP, ICC/IFmore details
Unsuitable for: IHC
Species reactivityReacts with: Human
Synthetic peptide within Human STAT6 aa 800-900 (C terminal). The exact sequence is proprietary.
- HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent staining of HeLa cells using ab32108
Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
Predicted band size: 94 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab32108 has not yet been referenced specifically in any publications.