Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E265] to STAT6
- Suitable for: WB, Flow Cyt, IP, ICC/IF
- Knockout validated
- Reacts with: Human
Product nameAnti-STAT6 antibody [E265]
See all STAT6 primary antibodies
DescriptionRabbit monoclonal [E265] to STAT6
Specificityab32108 recognises Stat-6. It does not cross react with other STAT family members
Tested applicationsSuitable for: WB, Flow Cyt, IP, ICC/IFmore details
Unsuitable for: IHC
Species reactivityReacts with: Human
Synthetic peptide within Human STAT6 aa 800-900 (C terminal). The exact sequence is proprietary.
- WB: HeLa and Raji cells. ICC/IF: Raji cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
Confocal image showing cytoplasmic staining in Raji cells.
Immunocytochemistry/immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labelling STAT6 with ab32108 at 10 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as a secondary antibody at 2 µg/mL. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
Predicted band size: 94 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32108 has not yet been referenced specifically in any publications.