Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

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Immunocytochemistry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
  • Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
  • Flow Cytometry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
  • Immunoprecipitation - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
  • Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
  • Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E265] to STAT6 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT6 antibody [E265] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [E265] to STAT6 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises Stat-6. It does not cross react with other STAT family members.

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, IPmore details
    Unsuitable for: IHC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human STAT6 aa 800-900 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Flow Cyt: HeLa cells IP: HeLa whole cell lysate.

  • General notes

    ab247230 is the carrier-free version of ab32108 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247230 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC.

    • Target

    • Function

      Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

    • Sequence similarities

      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.

    • Post-translational
      modifications

      Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

    • Cellular localization

      Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
    • Information by UniProt

    • Database links

      • Alternative names

        • 12S1644 antibody
        • D12S1644 antibody
        • IL 4 STAT antibody
        • IL-4 Stat antibody
        • IL4 STAT antibody
        • Interleukin 4 Induced antibody
        • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
        • Signal transducer and activator of transcription 6 antibody
        • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
        • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
        • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
        • STAT 6 antibody
        • STAT interleukin4 induced antibody
        • STAT, interleukin4 induced antibody
        • Stat6 antibody
        • STAT6_HUMAN antibody
        • STAT6B antibody
        • STAT6C antibody
        • Transcription factor IL 4 STAT antibody
        see all

      Images

      • Immunocytochemistry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Immunocytochemistry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        This data was developed using ab32108, the same antibody clone in a different buffer formulation.Confocal image showing cytoplasmic staining in Raji cells. Immunocytochemistry/immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labelling STAT6 with ab32108 at 10 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as a secondary antibody at 2 µg/mL. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
      • Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

        This data was developed using ab32108, the same antibody clone in a different buffer formulation.

        Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

        Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)

        Lane 3: HeLa whole cell lysate (20 µg)

        Lane 4: Raji whole cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.

        ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Flow Cytometry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Flow Cytometry - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        This data was developed using ab32108, the same antibody clone in a different buffer formulation.
        Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling STAT6 with purified ab32108 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
      • Immunoprecipitation - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Immunoprecipitation - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

        This data was developed using ab32108, the same antibody clone in a different buffer formulation.
        Purified ab32108 at 1/50 dilution (2µg) immunoprecipitating STAT6 in HeLa whole cell lysate.
        Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
        Lane 2 (+): ab32108 + HeLa whole cell lysate.
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32108 in HeLa whole cell lysate.
        VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
        Blocking Buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM/TBST.
        Observed band size: 100 kDa

      • Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Western blot - Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
        Predicted band size: 94 kDa
        Observed band size: 100 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab32108, the same antibody clone in a different buffer formulation.

         

      • Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)
        Anti-STAT6 antibody [E265] - BSA and Azide free (ab247230)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab247230? Please let us know so that we can cite the reference in this datasheet.

      ab247230 has not yet been referenced specifically in any publications.

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