RecombinantRabMAb

Recombinant Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

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Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18278-222] to STAT6 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18278-222] to STAT6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human STAT6 aa 600-700. The exact sequence is proprietary.
    Database link: P42226

  • General notes

    Ab251555 is the carrier-free version of ab217998. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251555 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab251555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 110, 81, 75 kDa (predicted molecular weight: 94 kDa).

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Images

  • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/1000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 5 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 6 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 7 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa
    Observed band size: 110,75,81 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab217998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 8 seconds; Lanes 4-5: 2 seconds; Lanes 6-7: 1 second.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.

  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    This data was developed using ab217998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID: 11781246] [PMID: 23575898]. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    This data was developed using ab217998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/2000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa
    Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?



    This data was developed using ab217998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 3 seconds; Lane 3: 5 seconds.

  • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

    This data was developed using ab217998, the same antibody clone in a different buffer formulation.

    STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate. 10 µg (Input).

    Lane 2: ab217998 IP in Raji whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

    This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.

  • Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
    Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251555? Please let us know so that we can cite the reference in this datasheet.

ab251555 has not yet been referenced specifically in any publications.

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