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RecombinantRabMAb

Recombinant Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

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Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
  • Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18278-222] to STAT6 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18278-222] to STAT6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251555 is the carrier-free version of ab217998.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 110, 81, 75 kDa (predicted molecular weight: 94 kDa).
Notes
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 110, 81, 75 kDa (predicted molecular weight: 94 kDa).

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

  • Database links

    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate
      Lane 4 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
      Lane 5 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
      Lane 6 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
      Lane 7 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 94 kDa
      Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?



      This data was developed using ab217998, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times: Lanes 1-3: 8 seconds; Lanes 4-5: 2 seconds; Lanes 6-7: 1 second.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.

    • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      This data was developed using ab217998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID: 11781246] [PMID: 23575898]. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
    • Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      Immunocytochemistry - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      This data was developed using ab217998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
    • Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      All lanes : Anti-STAT6 antibody [EPR18278-222] (ab217998) at 1/2000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Rat spleen tissue lysate
      Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 94 kDa
      Observed band size: 110,75,81 kDa why is the actual band size different from the predicted?



      This data was developed using ab217998, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 10 seconds; Lane 2: 3 seconds; Lane 3: 5 seconds.

    • Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

      This data was developed using ab217998, the same antibody clone in a different buffer formulation.

      STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate. 10 µg (Input).

      Lane 2: ab217998 IP in Raji whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 1 second.

      This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.

    • Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)
      Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (ab251555)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251555? Please let us know so that we can cite the reference in this datasheet.

    ab251555 has not yet been referenced specifically in any publications.

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