Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [YE361] to STAT6 (Alexa Fluor® 647)
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Product nameAnti-STAT6 antibody [YE361] (Alexa Fluor® 647)
See all STAT6 primary antibodies
DescriptionRabbit monoclonal [YE361] to STAT6 (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P42226
- ICC/IF: HACAT cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 488) (ab196478)
- Anti-STAT6 antibody [YE361] (HRP) (ab196480)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 594) (ab207012)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 405) (ab207013)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 555) (ab207014)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 568) (ab207015)
- Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)
- Anti-STAT6 antibody [YE361] (PE) (ab223917)
- Anti-STAT6 antibody [YE361] (FITC) (ab223918)
- Anti-STAT6 antibody [YE361] (APC) (ab223919)
- Anti-STAT6 antibody [YE361] (ab32520)
Our Abpromise guarantee covers the use of ab196928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
ab196928 staining STAT6 in HACAT cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196928 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196928 has not yet been referenced specifically in any publications.