Recombinant
RabMAb

Recombinant Anti-STAT6 antibody [YE361] (Allophycocyanin) (ab223919)

Overview

  • Product name

    Anti-STAT6 antibody [YE361] (Allophycocyanin)
    See all STAT6 primary antibodies
  • Description

    Rabbit monoclonal [YE361] to STAT6 (Allophycocyanin)
  • Host species

    Rabbit
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P42226

  • Positive control

    • Flow Cyt: NIH3T3 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Images

  • Overlay histogram showing NIH3T3 cells stained with ab223919 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223919, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in NIH3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab223919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223919.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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