Product nameAnti-STAT6 antibody [YE361] (FITC)
See all STAT6 primary antibodies
DescriptionRabbit monoclonal [YE361] to STAT6 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityThe antibody does not cross-react with other Stat family members.
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P42226
- ICC/IF: NIH3T3 cells. Flow Cyt: NIH3T3 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 488) (ab196478)
- Anti-STAT6 antibody [YE361] (HRP) (ab196480)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 647) (ab196928)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 594) (ab207012)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 405) (ab207013)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 555) (ab207014)
- Anti-STAT6 antibody [YE361] (Alexa Fluor® 568) (ab207015)
- Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)
- Anti-STAT6 antibody [YE361] (Phycoerythrin) (ab223917)
- Anti-STAT6 antibody [YE361] (Allophycocyanin) (ab223919)
- Anti-STAT6 antibody [YE361] (ab32520)
Our Abpromise guarantee covers the use of ab223918 in the following tested applications.
This product gave a positive signal in NIH3T3 cells fixed with 100% methanol (5 min)
FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Sequence similaritiesBelongs to the transcription factor STAT family.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
Overlay histogram showing NIH3T3 cells stained with ab223918 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223918, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
ab223918 staining STAT6 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223918 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab223918 has not yet been referenced specifically in any publications.