Product nameSTAT6 overexpression 293T lysate (whole cell)
DescriptionSTAT6 overexpression lysate
ab94186 is a 293T cell transfected lysate in which Human STAT6 has been transiently over-expressed using a pCMV-STAT6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. PTM: Tyrosine phosphorylated following stimulation by IL-4 and IL-3. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Our Abpromise guarantee covers the use of ab94186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94186 at 15 μg/lane on an SDS-PAGE gel
All lanes : Anti-STAT6 antibody (ab88540) at 1/500 dilution
Lane 1 :
STAT6 overexpression 293T lysate (whole cell) (ab94186)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94186 has not yet been referenced specifically in any publications.