Recombinant
RabMAb

Recombinant Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

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Overview

  • Product name

    Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22599-78] to STAT6 (phospho Y641) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, Flow Cyt, IP, WB, IHC-Pmore details
    Unsuitable for: ChIP or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Mouse STAT6 aa 600-700. The exact sequence is proprietary.
    Database link: P52633

  • Positive control

    • IHC-P: Human kidney tissue, Rat liver tissue and Mouse kidney tissue; WB: Daudi serum IL-4, 2.4G2 and RAW 264.7 serums starved for 24 hours, then treated with 100 ng/ml; IP: RAW 264.7 and Daudi lysates; FC: RAW 264.7 and Daudi cells.

  • General notes

    ab263950 is the carrier-free version of ab263947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.  

    ab263950 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab263950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.

 

 
Flow Cyt Use at an assay dependent concentration.

 

 
IP Use at an assay dependent concentration.

 

 
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
IHC-P 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ChIP or ICC/IF.

    • Target

    • Function

      Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

    • Sequence similarities

      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.

    • Post-translational
      modifications

      Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

    • Cellular localization

      Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (panel A), no staining after alkaline phosphatase treatment (panel B. PMID: 8085155, 16181056). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug with ab263947 at 1:30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263947 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:5000 dilution.

      Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

      Lane 2: ab263947 IP in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263947 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 2 min

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Dot blot analysis of STAT6 (phospho Y641) peptide (Lane 1 STAT6 non-phospho peptide peptide (Lane 2), labelling STAT 6 (pY641) with purified ab263947 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 26 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red)/Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse kidney (panel A), no staining after alkaline phosphatase treatment (panel B, PMID: 23155424). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug with ab263947 at 1:30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263947 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:5000 dilution.

      Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

      Lane 2: ab263947 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263947 in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 min

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat liver (panel A), no staining after alkaline phosphatase treatment (panel B). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    References

    ab263950 has not yet been referenced specifically in any publications.

    Publishing research using ab263950? Please let us know so that we can cite the reference in this datasheet.

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