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RecombinantRabMAb

Recombinant Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
  • Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22599-78] to STAT6 (phospho Y641) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB, Flow Cyt (Intra), IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22599-78] to STAT6 (phospho Y641) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.

  • Tested applications

    Suitable for: Dot blot, IP, WB, Flow Cyt (Intra), IHC-Pmore details
    Unsuitable for: ChIP or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue, Rat liver tissue and Mouse kidney tissue; WB: Daudi serum IL-4, 2.4G2 and RAW 264.7 serums starved for 24 hours, then treated with 100 ng/ml;IP: RAW 264.7 and Daudi lysates;Flow Cyt (intra): RAW 264.7 and Daudi cells.

  • General notes

    ab263950 is the carrier-free version of ab263947.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab263950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.

 

 
IP
Use at an assay dependent concentration.

 

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Dot blot
Use at an assay dependent concentration.

 

 
IP
Use at an assay dependent concentration.

 

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ChIP or ICC/IF.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt

  • Database links

    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (panel A), no staining after alkaline phosphatase treatment (panel B. PMID: 8085155, 16181056). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug with ab263947 at 1:30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263947 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:5000 dilution.

      Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

      Lane 2: ab263947 IP in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263947 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 2 min

      STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Dot blot analysis of STAT6 (phospho Y641) peptide (Lane 1 STAT6 non-phospho peptide peptide (Lane 2), labelling STAT 6 (pY641) with purified ab263947 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 26 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red)/Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730)isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse kidney (panel A), no staining after alkaline phosphatase treatment (panel B, PMID: 23155424). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug with ab263947 at 1:30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263947 at 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:5000 dilution.

      Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

      Lane 2: ab263947 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263947 in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 min

      STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling STAT6 (phospho Y641) with ab263947 at 1:5000 dilution (0.106 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat liver (panel A), no staining after alkaline phosphatase treatment (panel B). The section was incubated with ab263947 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947).

    • Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab263947 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      STAT6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263947). 

       

       

    • Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)
      Anti-STAT6 (phospho Y641) antibody [EPR22599-78] - BSA and Azide free (ab263950)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab263950? Please let us know so that we can cite the reference in this datasheet.

    ab263950 has not yet been referenced specifically in any publications.

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