Overview

  • Product name

  • Description

    Rabbit polyclonal to Statherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1-50 (MKFLVFAFIL ALMVSMIGAD SSEEKFLRRI GRFGYGYGPY QPVPEQPLYP) of Human Statherin (NP_003145).

  • Positive control

    • Human fetal thymus lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 7 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use at an assay dependent concentration. See Abreview.

Target

  • Function

    Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface.
  • Tissue specificity

    Secreted by parotid and submandibular glands.
  • Sequence similarities

    Belongs to the histatin/statherin family.
  • Post-translational
    modifications

    Substrate for transglutaminase-2. More than 95% of the cyclized peptide is cyclo-statherin Q-37, and less than 5% is cyclo-statherin Q-39. Cyclized forms account for about 1% of total statherin in saliva.
    Sulfated on tyrosine residues.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • STAT_HUMAN antibody
    • STATH antibody
    • Statherin antibody
    • Statherin precursor antibody
    • STR antibody
    see all

Images

  • Anti-Statherin antibody (ab97950) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 7 kDa



    Gel concentration: 10-20%
  • ab97950 Immunoprecipitating Stratherin, harvested synthetic protein from Human Tissue lysate. 20µg of Tissue lysate was incubated with primary antibody (1/200 in TBS buffer) and matrix (Protein G) for 24 hours at 4°C. For western blotting ab97950 (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

References

This product has been referenced in:

  • Algarni AA  et al. The impact of stannous, fluoride ions and its combination on enamel pellicle proteome and dental erosion prevention. PLoS One 10:e0128196 (2015). ELISA . Read more (PubMed: 26030135) »
  • Silva-Boghossian CM  et al. Quantitative proteomic analysis of gingival crevicular fluid in different periodontal conditions. PLoS One 8:e75898 (2013). ELISA ; Human . Read more (PubMed: 24098404) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Has this order already been placed?

If not then please include this code when ordering by phone or fax. If ordering from the website please include the code in the discounts field. This code will entitle you to one free primary antibody.

I hope this information is helpful. Please contact me if you have any questions.

Read More

Question
Answer

Below please find information regarding the new discount code and expiration date. I hope this is helpful. Please contact me again if you have any further questions.

======

I am very pleased to hear you would like to accept our offer and test ab97950 in IP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More
Application
Immunoprecipitation
Sample
Human Tissue lysate - other (Harvested synthetic protein)
Total protein in input
20 µg
Specification
Harvested synthetic protein
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Aug 03 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab97950 in IP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for contacting us.


Abcam does not currently have a recommneded protocol specifically for co-IP. I was able to find so information which may be valuable to you at a competators website (http://www.piercenet.com/browse.cfm?fldID=9C471132-0F72-4F39-8DF0-455FB515718F).




To our knowledge, ab97950 has not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab97950 now, test it in IP in human and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab97950 in IP. I will then send a discount code. This code must be issued before purchase so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab97950 turns out to be unsuitable forIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up