- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Stathmin 1 antibody
See all Stathmin 1 primary antibodies -
DescriptionGoat polyclonal to Stathmin 1
-
Host speciesGoat
-
Tested applicationsSuitable for: WB, IHC-Pmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
-
Immunogen
Synthetic peptide:
C-KNKESKDPADETEAD
, corresponding to C-terminal amino acids 135-149 of Human Stathmin 1. -
Positive control
- Human Testis Lysate, Human Thymus and Human Tonsil tissues
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 17 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. |
Target
-
FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
-
Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
-
Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain. -
Post-translational
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. -
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
-
Database links
- Entrez Gene: 616317 Cow
- Entrez Gene: 478175 Dog
- Entrez Gene: 3925 Human
- Entrez Gene: 16765 Mouse
- Entrez Gene: 29332 Rat
- Omim: 151442 Human
- SwissProt: Q3T0C7 Cow
- SwissProt: P16949 Human
see all -
Alternative names
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
see all
Images
-
Western blot - Anti-Stathmin 1 antibody (ab109986)Anti-Stathmin 1 antibody (ab109986) at 1 µg/ml + Human Testis Lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody (ab109986)Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab109986 at a concentration of 3.75 µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody (ab109986)Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab109986 at a concentration of 3.75 µg/ml.
References
ab109986 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"