Overview

  • Product name

  • Description

    Rabbit polyclonal to Stathmin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 23-27 (

    ILSPR

    ) of Human Stathmin 1 (UniProt P16949).

  • Positive control

    • HeLa cells; 293 and HeLa cell extracts; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • Tissue specificity

    Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • Sequence similarities

    Belongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • Post-translational
    modifications

    Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Stathmin 1 antibody (ab131481) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : HeLa cell extract

    Predicted band size: 17 kDa

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling Stathmin 1 with ab131481 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Stathmin 1 with ab131481 at 1/50 dilution (left) or ab131481 preincubated with blocking peptide (right).

References

This product has been referenced in:

  • Wang Y  et al. S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells. Oncotarget 8:111866-111881 (2017). Read more (PubMed: 29340097) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Dentate gyrus)
Permeabilization
Yes - TritonX-100(0.05%)
Specification
Brain, Dentate gyrus
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 27 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up