Anti-Stathmin 1 antibody [EP1572] (ab119991)
Key features and details
- Rabbit monoclonal [EP1572] to Stathmin 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Stathmin 1 antibody [EP1572]
See all Stathmin 1 primary antibodies -
Description
Rabbit monoclonal [EP1572] to Stathmin 1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Human spleen, Jurkat and SH-SY5Y cell lysates; Human brain tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1572 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
Notes |
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WB
1/5000 - 1/20000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. -
Tissue specificity
Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. -
Sequence similarities
Belongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain. -
Post-translational
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 3925 Human
- Entrez Gene: 16765 Mouse
- Entrez Gene: 29332 Rat
- Omim: 151442 Human
- SwissProt: P16949 Human
- SwissProt: P54227 Mouse
- SwissProt: P13668 Rat
- Unigene: 209983 Human
see all -
Alternative names
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
see all
Images
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All lanes : Anti-Stathmin 1 antibody [EP1572] (ab119991) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human spleen lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (ab119991)
ab119991 at 1/100 dilution staining Stathmin 1 in Paraffin-embedded Human Brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab119991 has not yet been referenced specifically in any publications.