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    stathmin-1-antibody-ep1573y-ab52630.pdf

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RecombinantRabMAb

Recombinant Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

  • Datasheet
Reviews (5) Submit a question References (28)

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Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
  • Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1573Y] to Stathmin 1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE

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Overview

  • Product name

    Anti-Stathmin 1 antibody [EP1573Y]
    See all Stathmin 1 primary antibodies
  • Description

    Rabbit monoclonal [EP1573Y] to Stathmin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Stathmin 1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P16949

  • Positive control

    • WB: Human lymph node, Mouse brain, PC-12 whole cell lysates IP: human colon and HeLa cells. IHC-P: lymph node, Human lung carcinoma, Mouse brain, and Rat brain tissue. Flow Cyt (intra): Jurkat cells ICC/IF: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1573Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Stathmin 1 antibody [EP1573Y] (ab199735)
    • Alexa Fluor® 647 Anti-Stathmin 1 antibody [EP1573Y] (ab199812)
    • Alexa Fluor® 594 Anti-Stathmin 1 antibody [EP1573Y] (ab215094)
    • Anti-Stathmin 1 antibody [EP1573Y] - BSA and Azide free (ab221017)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Stathmin 1 protein (ab87492)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/100

ICC/IF
1/100 - 1/250.
WB (2)
1/10000. Detects a band of approximately 19 kDa.

For unpurified use at 1/50000

IP (1)
1/30.

For unpurified use at 1/50

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols

Notes
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/100

ICC/IF
1/100 - 1/250.
WB
1/10000. Detects a band of approximately 19 kDa.

For unpurified use at 1/50000

IP
1/30.

For unpurified use at 1/50

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols

Target

  • Function

    Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • Tissue specificity

    Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • Sequence similarities

    Belongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • Post-translational
    modifications

    Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P16949 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3925 Human
    • Entrez Gene: 16765 Mouse
    • Entrez Gene: 29332 Rat
    • Omim: 151442 Human
    • SwissProt: P16949 Human
    • SwissProt: P54227 Mouse
    • SwissProt: P13668 Rat
    • Unigene: 209983 Human
    • Unigene: 378957 Mouse
    • Unigene: 555 Rat
    see all
  • Alternative names

    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

Images

  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/50000 dilution (Purified) + Mouse brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/10000 dilution (Purified) + Human lymph node lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Stathmin 1 with purified ab52630 at 1/200 dilution (3.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Western blot - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/500000 dilution + PC12 lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Observed band size: 18 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    ab52630 (purified) at 1/30 dilution (2ug) immunoprecipitating Stathmin 1 in HeLa whole cell lysates.
    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab52630 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52630 in HeLa whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab52630 at 1/60 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat brain tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse brain tissue sections labeling Stathmin 1 with purified ab52630 at 1/2000 dilution (0.31 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    ab52630 stained HeLa cells
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    ab52630, at a 1/250 dilution, staining human Stathmin 1 in lymph node tissue, using Immunohistochemistry, Paraffin embedded sections.
  • Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Flow Cytometry (Intracellular) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

    Overlay histogram showing Jurkat cells stained with ab52630 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52630, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

     

  • Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Immunoprecipitation - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)Image courtesy of Qifeng Lin by Abreview.
    Whole cell lysate prepared from human colon cells was loaded at 20µg. The immunoprecipitation step was performed using Protein A/G. ab52630 used at a 1/200 dilution for 12 hours at 4°C. For WB ab52630 used at a 1/10000 dilution.

    See Abreview

  • Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
    Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (28)

Publishing research using ab52630? Please let us know so that we can cite the reference in this datasheet.

ab52630 has been referenced in 28 publications.

  • Ju WT  et al. Stathmin guides personalized therapy in oral squamous cell carcinoma. Cancer Sci 111:1303-1313 (2020). PubMed: 31994271
  • Jia L  et al. Long noncoding RNA TPT1-AS1 downregulates the microRNA-770-5p expression to inhibit glioma cell autophagy and promote proliferation through STMN1 upregulation. J Cell Physiol 235:3679-3689 (2020). PubMed: 31637705
  • Morris EJ  et al. A Model of Differential Mammary Growth Initiation by Stat3 and Asymmetric Integrin-a6 Inheritance. Cell Rep 30:3605-3615.e5 (2020). PubMed: 32187533
  • Zhang J  et al. Amentoflavone triggers cell cycle G2/M arrest by interfering with microtubule dynamics and inducing DNA damage in SKOV3 cells. Oncol Lett 20:168 (2020). PubMed: 32934735
  • Wen XM  et al. Zyxin (ZYX) promotes invasion and acts as a biomarker for aggressive phenotypes of human glioblastoma multiforme. Lab Invest 100:812-823 (2020). PubMed: 31949244
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Stathmin 1 antibody [EP1573Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (primary keratinocytes)
Gel Running Conditions
Reduced Denaturing (any kD BioRad)
Loading amount
50 µg
Specification
primary keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Julien Coutier

Verified customer

Submitted Jul 02 2018

Western blot abreview for Anti-Stathmin 1 antibody [EP1573Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT-116)
Loading amount
20 µg
Specification
HCT-116
Gel Running Conditions
Non-reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Qifeng Lin

Verified customer

Submitted Jan 13 2011

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