Recombinant Anti-Stathmin 1 antibody [SP49] (ab101690)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP49] to Stathmin 1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Stathmin 1 antibody [SP49]
See all Stathmin 1 primary antibodies -
Description
Rabbit monoclonal [SP49] to Stathmin 1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig -
Immunogen
Synthetic peptide within Human Stathmin 1 aa 100 to the C-terminus (internal sequence). The exact sequence is proprietary.
Database link: P16949 -
Positive control
- IHC-P: Human Tonsil, and breast carcinoma tissue; WB: NIH3T3 cell lysate; Flow Cyt (Intra): Jurkat and Neuro-2a cell lysates; ICC: Jurkat cell lysate.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP49 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
In details: boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed bycooling at RT for 20 min. |
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ICC/IF |
1/500.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. In details: boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed bycooling at RT for 20 min. |
ICC/IF
1/500. |
Target
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Function
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. -
Tissue specificity
Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. -
Sequence similarities
Belongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain. -
Post-translational
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 616317 Cow
- Entrez Gene: 478175 Dog
- Entrez Gene: 3925 Human
- Entrez Gene: 16765 Mouse
- Entrez Gene: 29332 Rat
- Omim: 151442 Human
- SwissProt: Q3T0C7 Cow
- SwissProt: P16949 Human
see all -
Alternative names
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Stathmin 1 with Purified ab101690 at 1/400 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-Stathmin 1 antibody [SP49] (ab101690) at 1/25 dilution + lysate prepared from NIH3T3 cells
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab101690 at 1/500 (0.6 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of HepG2 (human liver hepatocellular carcinoma cell line) labeling Stathmin 1 with purified ab101690 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
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Flow cytometry analysis of Jurkat (human acute T cell leukemia) labeling Stathmin 1 with purified ab101690 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
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ab101690 at 1/100 dilution staining Stathmin 1 in formalin-fixed, paraffin-embedded Human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Flow cytometric analysis of rabbit anti-Stathmin 1 (SP49) antibody ab101690 in HeLa cells(green) compared to negative control of rabbit IgG (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab101690 has been referenced in 2 publications.
- Pepe G et al. Reduced lymphotoxin-beta production by tumour cells is associated with loss of follicular dendritic cell phenotype and diffuse growth in follicular lymphoma. J Pathol Clin Res 4:124-134 (2018). PubMed: 29665320
- Ouyang H et al. Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Front Physiol 8:281 (2017). WB . PubMed: 28533755