Key features and details
- Rabbit polyclonal to Stathmin 1 (phospho S16)
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Stathmin 1 (phospho S16) antibody
See all Stathmin 1 primary antibodies
DescriptionRabbit polyclonal to Stathmin 1 (phospho S16)
SpecificityThis antibody detects endogenous levels of Stathmin 1 only when phosphorylated at serine 16. (Human: Ser16; Mouse: Ser16; Rat: Ser16).
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Stathmin 1. synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 16 (R-A-SP-G-Q)
Database link: P16949
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab47328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain.
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
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ab47328 has been referenced in 8 publications.
- You M et al. Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny. Chemosphere 211:758-766 (2018). PubMed: 30099160
- Chen X et al. The Cdc2/Cdk1 inhibitor, purvalanol A, enhances the cytotoxic effects of taxol through Op18/stathmin in non-small cell lung cancer cells in vitro. Int J Mol Med 40:235-242 (2017). PubMed: 28534969
- Alesi GN et al. RSK2 signals through stathmin to promote microtubule dynamics and tumor metastasis. Oncogene 35:5412-5421 (2016). PubMed: 27041561
- Kempf SJ et al. An integrated proteomics approach shows synaptic plasticity changes in an APP/PS1 Alzheimer's mouse model. Oncotarget 7:33627-48 (2016). PubMed: 27144524
- Zhang L et al. Increased stathmin expression strengthens fear conditioning in epileptic rats. Biomed Rep 3:28-32 (2015). PubMed: 25469242
- Yip YY et al. cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin phosphorylation for the maintenance of interphase microtubules during osmotic stress. J Biol Chem 289:2157-69 (2014). PubMed: 24302736
- Tian X et al. Novel role of stathmin in microtubule-dependent control of endothelial permeability. FASEB J 26:3862-74 (2012). PubMed: 22700873
- Higuero AM et al. Kidins220/ARMS modulates the activity of microtubule-regulating proteins and controls neuronal polarity and development. J Biol Chem 285:1343-57 (2010). WB, ICC/IF ; Rat . PubMed: 19903810