Product nameAnti-Stathmin 1 (phospho S24) antibody [EP2124Y]
See all Stathmin 1 primary antibodies
DescriptionRabbit monoclonal [EP2124Y] to Stathmin 1 (phospho S24)
SpecificityThis antibody detects Stathmin 1 phosphorylated at Serine 24.
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Phospho specific peptide corresponding to residues surrounding Serine 24 of human Stathmin 1.
- HeLa cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab62336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).|
|ICC||1/100 - 1/250.|
FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain.
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
All lanes : Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (ab62336) at 1/1000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with FBS+Calyculin A)
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
The bottom image shows beta Tubulin, included as a positive control.
This product has been referenced in:
- Alesi GN et al. RSK2 signals through stathmin to promote microtubule dynamics and tumor metastasis. Oncogene 35:5412-5421 (2016). Read more (PubMed: 27041561) »
- Zhao E et al. Stathmin mediates hepatocyte resistance to death from oxidative stress by down regulating JNK. PLoS One 9:e109750 (2014). WB . Read more (PubMed: 25285524) »