Overview

  • Product name

    Anti-Stathmin 1 (phospho S24) antibody [EP2124Y]
    See all Stathmin 1 primary antibodies
  • Description

    Rabbit monoclonal [EP2124Y] to Stathmin 1 (phospho S24)
  • Host species

    Rabbit
  • Specificity

    This antibody detects Stathmin 1 phosphorylated at Serine 24.
  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Serine 24 of human Stathmin 1.

  • Positive control

    • HeLa cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab62336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
    • Tissue specificity

      Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
    • Sequence similarities

      Belongs to the stathmin family.
      Contains 1 SLD (stathmin-like) domain.
    • Post-translational
      modifications

      Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • C1orf215 antibody
      • Lag antibody
      • LAP 18 antibody
      • LAP18 antibody
      • Leukemia associated phosphoprotein p18 antibody
      • Leukemia-associated phosphoprotein p18 antibody
      • Metablastin antibody
      • Oncoprotein 18 antibody
      • OP 18 antibody
      • Op18 antibody
      • p18 antibody
      • p19 antibody
      • Phosphoprotein 19 antibody
      • Phosphoprotein p19 antibody
      • pp17 antibody
      • pp19 antibody
      • PR22 antibody
      • Pr22 protein antibody
      • Prosolin antibody
      • Protein Pr22 antibody
      • SMN antibody
      • Stathmin antibody
      • Stathmin1 antibody
      • STMN 1 antibody
      • Stmn1 antibody
      • STMN1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (ab62336) at 1/1000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with FBS+Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 17 kDa
      Observed band size: 19 kDa
      why is the actual band size different from the predicted?



      The bottom image shows beta Tubulin, included as a positive control.

    References

    This product has been referenced in:

    • Alesi GN  et al. RSK2 signals through stathmin to promote microtubule dynamics and tumor metastasis. Oncogene 35:5412-5421 (2016). Read more (PubMed: 27041561) »
    • Zhao E  et al. Stathmin mediates hepatocyte resistance to death from oxidative stress by down regulating JNK. PLoS One 9:e109750 (2014). WB . Read more (PubMed: 25285524) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Q&A

    Question
    Answer

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

    Credit ID: #####

    As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

    I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

    Read More

    Answer

    Thank you for your message and for providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time they have spent on these experiments, it is disappointing this has not worked for the customer. I would like to offer a refund or credit note in compensation (providing the product has been purchased in the last 6 months). In order to arrange this, I would appreciate if you could confirm the order number and date of purchase?

    Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Answer

    Thank you for your message.

    To confirm:

    1. Growing cells were starved for 24hr in the medium without FBS.

    2. Growing cells treated the with 20% FBS for 3hr.

    3. Add calyculin A to the growing cells at the concentration of 100ng/mln for 30 minutes at 37oC.

    4. After the 30 min incubation, the cells were harvested and lysed with RIPA buffer containing the usual protease inhibitor cocktail.

    I hope this will be helpful. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Answer

    Thank you for your reply and for kindly providing this information.

    Reviewing the details, I can suggest my main concern is still that the cells have not been treated.

    Here is the treatment which was used in testing the cells (as shown in the WB image on the datasheet, HeLa):

    1. The cells were starved for 24hr in the medium without FBS.

    2. Treated the cells with 20% FBS for 3h. 3.

    Added calyculin A at the concentration of 100ng/mln.

    4. After 30min, the cells were harvested and lysed.

    I hope this will be helpful. Please do not hesitate to let me know the results. I hope we can resolve this case as soon as possible.

    Read More

    Question

    Product code: 62336
    Lot number: GR69244-2

    Inquiry: Dear Tech Support Team, Please read below the details of customer's complaint. Please advise. Thanks in advance. )
    Abcam product code ab62336
    2) Abcam order reference number or product batch number lot # GR-69244-2
    3) Description of the problem - Did not recognize protein in 3 cell types including recommended positive control (NSC34, Cos7, Hela)
    4) Sample preparation: Type of sample: whole cell lysates Lysis buffer : 1% triton / 1% NP40. Protease inhibitors: complete (prot. inhibitor cocktail by Roche) Phosphatase inhibitors : Phos-stop Reducing agent β-ME
    Boiling for ≥5 min? yes/no
    Protein loaded ug/lane or cells/lane – 30-50ug.
    Positive control Hela cell lysates
    Negative control
    5) Percentage of gel 12%, 10%
    Type of membrane nitrocellulose Protein transfer verified Yes – marker, ponceu
    Blocking agent and concentration 5% milk in TBST Blocking time 1hr Blocking temperature room temp’
    6) Primary antibody (If more than one was used, describe in “additional notes”) : Concentration or dilution 1:200, 1:1000, 1:500 Diluent buffer 5% milk Incubation time Over night Incubation temperature: 4°C
    7) Secondary antibody: Species: Donkey Reacts against: rabbit Concentration or dilution 1:1000 Diluent buffer milk / TBST Incubation time 1hr Incubation temperature: room temp. Fluorochrome or enzyme conjugate: HRP
    8) Washing after primary and secondary antibodies: Buffer TBST Number of washes 3 X 5’
    9)Detection method
    10) How many times have you run this staining? 3 Do you obtain the same results every time? yes What steps have you altered to try and optimize the use of this antibody? cell type, dilution, ug protein. Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem. Nothing on the film. other membranes in the same exposure did give results. Half of the same membrane incubated with a different Ab did give result.
    Have a nice day.

    Read More
    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I would like to reassure you that ab62336 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

    Reviewing this case, I would if you can confirm some further details in order to help me investigate this further:

    1. Please confirm further details of the results. Was the membrane completely clear, or was there background staining, or non specific bands at incorrect size? I would appreciate if you are able to provide an image, including molecular weight markers, which will help us to assess the results.

    2. The antibody detects the phosphorylated (S24) stathmin 1. As far as I am aware, the phosphorylation can require inducing. For example, the HeLa cell lysate used in the WB shown on the datasheet has been treated with FBS+Calyculin A. Could you confirm if the cells used in these experiments have been treated? I can suggest considering a further literature search for more information.

    3. Has the transfer to the membrane and quality of the sample been assessed using a loading control.

    I hope this information is helpful. Please do not hesitate to contact me again with the further requested details and I hope we can resolve this case as soon as possible.

    Read More

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